Potential anti-biofilm activity of human enteric Lactobacillus acidophilus on Urophaogenic E.coli (UPEC)‎ biofilm formation ability

الملخص EN

It has been suggest that biofilm formation by uropathogenic E.coli (UPEC) is associated with recurrence and persistence of urinary tract infections (UTIs).

The conventional strategies of antibiotic therapy not only proved to be sub – optimal efficacy but also led to the development of multi – drug resistant strains of UPEC.

One promising alternative therapy is the use of certain probiotics particularly Lactobacillus spp.

to prevent and treat recurrent complicated and uncomplicated UTIs.

The present study evaluated the antagonistic and anti – biofilm activities of eighteen human derived enteric and vaginal Lactobacillus isolates on multi – drug biofilm producer UPEC, isolated from recurrent UTI..

Eight isolates of assessed Lactobacillus isolates were showed clear inhibitory actions against UPEC planktonic cells, with average of 8 – 18mm diameter of inhibition zones, the enteric isolate L acidophilus Lf1 showed the largest inhibition zone in agar – well diffusion technique.

Significant (P<0.05) reduction of biofilm formation ability was proved in UPEC when treated by L.

acidophilus (Lf1) crude cell free supernatant (CFS), with about seven- folds reduction in biofilms densities .

The neutralized and HR2ROR2R excluded CFSs of Lf1 also exhibited well anti – biofilm activity, the HR2ROR2R excluded CFS was displayed superior reduction in biofilm formation abilities in that the percentage reduction values average was of about 75- 83 %, While neutralized CFS pronounced slightly less reduction percentage against biofilms, with an average values of about 55 -64 % .

Bacteriocins was the most potent anti – biofilm agent , the 80% saturation fraction of Lf1 bacteriocins was pronounced the higher inhibitory potential on biofilms abilities of UPEC, as biofilm reduction values average was of about 80.5-83.5%