Aquaporin 8 regulates mitochondrial glycogen phosphorylase expression in C57BL6 mice

الملخص EN

Aquaporins (AQPs) are group of membranous water channel proteins that regulates water and small molecule movements through cell membrane.

Among this group, aquaporin 8 which express in high abundance in colon, liver and kidneys.

With the generation of aquaporin 8 knockout model, C57BL6 mice hepatic tissues were fractionated to purify mitochondrial fractions in both wild and AQP8 knockout model.

Tandem mass spectrometric analysis revealed a significant downregulation of glycogen phosphorylase in AQP8 knockout mice.

This finding was supported by western blotting.

Immune-gold electron microscopy showed mitochondrial cisternae localization of glycogen phosphorylase in higher abundance than in knockout model.

Present findings suggest a novel strong association between aquaporin 8 and mitochondrial glycogen phosphorylase which pinpoints indirect involvement of AQP8 in glycogen metabolism.