Spectrophotometric determination of copper (II)‎ using 2,2 (O-Tolidine-4,4-bis azo)‎ bis (4,5-diphenyl imidazole)‎ (MBBAI)‎

الملخص EN

Spectrophotometric method was developed for the determination of copper(II) ion.

Synthesized (2,2[O-Tolidine-4,4-bis azo]bis[4,5-diphenyl imidazole]) (MBBAI) was used as chromogenic reagent at pH=5.

Various factors affecting complex formation, such as, pH effect, reagent concentration, time effect and temperature effect, have been considered and studied.

Under optimum conditions concentration ranged from (5.00-80.00) µg/mL of copper(II) obeyed Beer`s Low.

Maximum absorption of the complex was 409nm with molar absorpitivity 0.127x104 L mol-1 cm-1.

Limit of detection(LOD) and Limit of quantification were 1.924 and 6.42 μg/mL, respectively.

The stoichiometric composition of the chelate is 1:2 (Cu:MBBAI).

Experimental results for studying some selected ions as interference were reported.

The developed method was successfully applied to determine copper (II) ion in dental filling.