Shoot multiplication of Pear cv. Othmany as affected by sugar type and nutrient media in vitro

الملخص EN

The effect of adding three types of sugars (sucrose, Glucose or mannitol) at concentration of 30 g.

L-1 each to MS [13] or WPM [11] media and the effect of nutrient Conc.

of MS and WPM in (half strength, full strength, full and half strength and double strength of nutrient)contain 2.

5 mg.

L-1 BA and 0.

25 mg.

L-1 IBA, on shoot multiplication of the pear plants cv.

Othmany were studied.

The results after eight weeks of multiplication, MS medium achieved the best result in all the studying characters, No.

of shoots (3.

70), shoot length (2.

63 cm) and the No.

of leaves (27.

33).

However, the sucrose had a significant effect on No.

of shoots and No.

of leaves (5.

39 and 40.

28) respectively, but the longer shoot was achieved in adding Glucose to the medium, which differs significantly from Mannitol.

The effect of concentration of Nutrient media on shoots multiplication, the results showed that the higher No.

of Shoots and leaves were achieved in WPM (4.

81 and 41.

54) respectively that not differ significantly of MS.

As soon as the best significantly length of shoots (3.

46 cm) was achieved in MS in comparison with WPM.