Evaluation of the effect of flavonoid extract of Ginkgo Biloba leaves and glimephan on oxidative stress and retina degeneration in diabetic male rabbits induced by streptozotocin

الملخص EN

This study was carried out in animal house of Collage of Veterinary Medicine/ University of Basrah.

An attempt has been done to induce diabetic by streptozotocin in male rabbits and investigation of the diabetes was induced oxidative stress and retina degeneration.

Moreover, the purpose of this study was to isolate and evaluate the ameliorating effect of flavonoid extract of Ginkgo biloba leaves and glimephan in the prophylaxes or delay the development of diabetic retina degeneration and scavenging free radical induced oxidative stress and diabetic retina degeneration in male rabbits.

The study was done on (32) adult male rabbits, their weight ranged between (2000-2500g) and aged between 7-8 months.

The male rabbits were divided randomly into four groups, each group consists of eight rabbits as the following: Group1: - Male rabbits at (Negative controls(-ve)) administrated normal saline1ml orally for 30 days.

Group2: - Male rabbits at(Positive control(+ve)) were given streptozotocin (65mg/kg B.

W.

dissolve in sodium citrate I.

V.

) for two days and remain for 30 days.

Group3: - Male rabbits were given streptozotocin( 65mg/kg B.

W.

dissolve in sodium citrate I.

V.

) for two days, then treated with flavonoid extract of Ginkgo biloba leave (500mg/kg B.

W.

orally administration) for 30days.

Group4: - Male rabbits were given streptozotocin (65mg/kg B.

W.

dissolve in sodium citrate I.

V.

) for two days, then treated with glimphan drug (0.

1mg/kg orally administration) for 30 days.

At the end of the experimental period, the blood samples were collected from heart by cardiac puncture for isolated serum and analysis biochemical parameters such as glucose, insulin, malonaldehyd (MDA), Superoxide dismutase (SOD) and glutathione peroxidase (GPx) concentrations and lipid profile.

The results revealed a significant (P≤0.

05) decrease of body weight, body weight gain and HDL concentrations in serum diabetic rabbits control(+ve) compared with control(-ve) while the results showed a significant (P≤0.

05) increase the glucose, insulin cholesterol LDL, VLDL, MDA and GPx concentrations in serum diabetic male rabbits control(+ve) compared with control(-ve).

The results obtained a significant decrease (P≤0.

05) in body weight, body weight gain, SOD and HDL concentrations in serum diabetic male rabbits control(+ve).

While male rabbits treated with flavonoid extract of Ginkgo biloba the results observed a significant (P≤0.

05) increase in body weight and body weight gain compared with control(+ve).

Whereas the results were revealed a significant(P≤0.

05)decrease of glucose, insulin, cholesterol, LDL, VLDL.

MDA and GPx concentration rabbits treated with flavonoid extract of Ginkgo biloba leave compared with control(+ve) and glimephan also in addition, this extract improved the retina degeneration.

Histological examination observed many pathological changes in pancreas and retina in diabetic group but in treated with flavonoid extract of Ginkgo biloba, the histological changes were near to the normal status.

It is concluded that good anti-diabetic activity, hypoglycemia effect and regeneration of retina.

Based on these results, we suggested the possible utilization of Ginkgo biloba as a therapy to prevent diabetic complication and improved the retina degeneration compared with another treated such as glimephan drugs