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CFP and YFP, but Not GFP, Provide Stable Fluorescent Marking of Rat Hepatic Adult Stem Cells
المؤلفون المشاركون
Taghizadeh, Rouzbeh R.
Sherley, James L.
المصدر
العدد
المجلد 2008، العدد 2008 (31 ديسمبر/كانون الأول 2008)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2008-03-02
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
The stable expression of reporter genes in adult stem cells (ASCs) has important applications in stem cell biology.
The ability to integrate a noncytotoxic, fluorescent reporter gene into the genome of ASCs with the capability to track ASCs and their progeny is particularly desirable for transplantation studies.
The use of fluorescent proteins has greatly aided the investigations of protein and cell function on short-time scales.
In contrast, the obtainment of stably expressing cell strains with low variability in expression for studies on longer-time scales is often problematic.
We show that this difficulty is partly due to the cytotoxicity of a commonly used reporter, green fluorescent protein (GFP).
To avoid GFP-specific toxicity effects during attempts to stably mark a rat hepatic ASC strain and, therefore, obtain stable, long-term fluorescent ASCs, we evaluated cyan fluorescent protein (CFP) and yellow fluorescent protein (YFP), in addition to GFP.
Although we were unable to derive stable GFP-expressing strains, stable fluorescent clones (up to 140 doublings) expressing either CFP or YFP were established.
When fluorescently marked ASCs were induced to produce differentiated progeny cells, stable fluorescence expression was maintained.
This property is essential for studies that track fluorescently marked ASCs and their differentiated progeny in transplantation studies.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Taghizadeh, Rouzbeh R.& Sherley, James L.. 2008. CFP and YFP, but Not GFP, Provide Stable Fluorescent Marking of Rat Hepatic Adult Stem Cells. BioMed Research International،Vol. 2008, no. 2008, pp.1-9.
https://search.emarefa.net/detail/BIM-987767
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Taghizadeh, Rouzbeh R.& Sherley, James L.. CFP and YFP, but Not GFP, Provide Stable Fluorescent Marking of Rat Hepatic Adult Stem Cells. BioMed Research International No. 2008 (2008), pp.1-9.
https://search.emarefa.net/detail/BIM-987767
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Taghizadeh, Rouzbeh R.& Sherley, James L.. CFP and YFP, but Not GFP, Provide Stable Fluorescent Marking of Rat Hepatic Adult Stem Cells. BioMed Research International. 2008. Vol. 2008, no. 2008, pp.1-9.
https://search.emarefa.net/detail/BIM-987767
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-987767
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
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