Evaluation of Bcl-2 Expression and Caspase-3 in STZ-Induced Diabetic Rats

المؤلفون المشاركون

Jafari Anarkooli, Iraj
Varasteh, Abdol-ReZa
Sankian, Mojtaba
Haghir, Hossein
Ahmadpour, Shahriar

المصدر

Journal of Diabetes Research

العدد

المجلد 2008، العدد 2008 (31 ديسمبر/كانون الأول 2008)، ص ص. 1-6، 6ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2008-07-14

دولة النشر

مصر

عدد الصفحات

6

التخصصات الرئيسية

الأمراض
الطب البشري

الملخص EN

We assessed the expression of Bcl-2 family members at both mRNA and protein levels as well as the Caspase-3 activity, in order to investigate the occurrence of apoptosis in hippocampus of STZ-induced diabetic rats.

We selected twenty-four Wistar rats; half of them were made diabetic by intraperitoneal injection of a single 60 mg/kg dose of streptozotocin (STZ, IP), while the others received normal saline and served as controls.

The expressions of Bcl-2, Bcl-xL, and Bax mRNA and proteins were measured using RT-PCR and western blotting, respectively.

Caspases-3 activity was determined by using the Caspase-3/CPP32 Fluorometric Assay Kit.

The result showed that mRNA and protein levels of Bcl-2 and Bcl-xL were lower in hippocampus of diabetic group than that of the control group, whereas expressions of Bax in hippocampus of diabetic rats were higher than that of controls at both mRNA and protein levels (P<.01).

Hyperglycemia was found to raise 6.9-fold hippocampal caspase-3 activity in diabetic group compared with control group (P<.001).

Therefore, the induction of diabetes is associated with increased ratios of Bax/Bcl-2, Bax/Bcl-xL, and increased caspase-3 activity in hippocampus which shows that apoptosis is favored in hippocampal region.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Jafari Anarkooli, Iraj& Sankian, Mojtaba& Ahmadpour, Shahriar& Varasteh, Abdol-ReZa& Haghir, Hossein. 2008. Evaluation of Bcl-2 Expression and Caspase-3 in STZ-Induced Diabetic Rats. Journal of Diabetes Research،Vol. 2008, no. 2008, pp.1-6.
https://search.emarefa.net/detail/BIM-988015

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Jafari Anarkooli, Iraj…[et al.]. Evaluation of Bcl-2 Expression and Caspase-3 in STZ-Induced Diabetic Rats. Journal of Diabetes Research No. 2008 (2008), pp.1-6.
https://search.emarefa.net/detail/BIM-988015

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Jafari Anarkooli, Iraj& Sankian, Mojtaba& Ahmadpour, Shahriar& Varasteh, Abdol-ReZa& Haghir, Hossein. Evaluation of Bcl-2 Expression and Caspase-3 in STZ-Induced Diabetic Rats. Journal of Diabetes Research. 2008. Vol. 2008, no. 2008, pp.1-6.
https://search.emarefa.net/detail/BIM-988015

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-988015