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Real-Time Monitoring of Apoptosis by Caspase-3-Like Protease Induced FRET Reduction Triggered by Amyloid Aggregation
المؤلفون المشاركون
Köhler, Martin
Berggren, Per-Olof
Schultz, Sebastian W.
Leibiger, Ingo
Paulsson, Johan F.
Westermark, Gunilla T.
المصدر
العدد
المجلد 2008، العدد 2008 (31 ديسمبر/كانون الأول 2008)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2008-05-06
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Amyloid formation is cytotoxic and can activate the caspase cascade.
Here, we monitor caspase-3-like activity as reduction of fluorescence resonance energy transfer (FRET) using the contstruct pFRET2-DEVD containing enhanced cyan fluorescent protin (EYFP) linked by the caspase-3 specific cleavage site residues DEVD.
Beta-TC-6 cells were transfected, and the fluoorescence was measured at 440 nm excitation and 535 nm (EYFP) and 480 nm (ECFP) emission wavelength.
Cells were incubated with recombinant pro lset Amyloid Polypeptide (rec prolAPP) or the processing metabolites of prolAPP; the N-terminal flanking peptide withIAPP (recN+IAPP); IAPP with the C-terminal flanking peptied (recIAPP+C) and lslet Amyloid Polypeptide (recIAPP) .
Peptides were added in solubilized from (50 μM) or as performed amyloid-like fibrils, or as a combination of these.
FRET was measured and incubation with a mixture of solubilized peptide and performed fibrils resulted in loss of FRET and apoptosis was determined to occure in cells incubated with recproIAPP (49%), recN+IAPP (46%), recIAPP (72%) and recIAPP+C (59%).
These results show that proIAPP and the processing intermediates reside the same cell toxic capacity as IAPP, and they can all have a central role in the reduction of beta-cell number in type 2 diabetes.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Paulsson, Johan F.& Schultz, Sebastian W.& Köhler, Martin& Leibiger, Ingo& Berggren, Per-Olof& Westermark, Gunilla T.. 2008. Real-Time Monitoring of Apoptosis by Caspase-3-Like Protease Induced FRET Reduction Triggered by Amyloid Aggregation. Journal of Diabetes Research،Vol. 2008, no. 2008, pp.1-12.
https://search.emarefa.net/detail/BIM-988025
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Paulsson, Johan F.…[et al.]. Real-Time Monitoring of Apoptosis by Caspase-3-Like Protease Induced FRET Reduction Triggered by Amyloid Aggregation. Journal of Diabetes Research No. 2008 (2008), pp.1-12.
https://search.emarefa.net/detail/BIM-988025
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Paulsson, Johan F.& Schultz, Sebastian W.& Köhler, Martin& Leibiger, Ingo& Berggren, Per-Olof& Westermark, Gunilla T.. Real-Time Monitoring of Apoptosis by Caspase-3-Like Protease Induced FRET Reduction Triggered by Amyloid Aggregation. Journal of Diabetes Research. 2008. Vol. 2008, no. 2008, pp.1-12.
https://search.emarefa.net/detail/BIM-988025
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-988025
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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