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Characterization of Multidrug Resistant ESBL-Producing Escherichia coli Isolates from Hospitals in Malaysia
المؤلفون المشاركون
Thong, Kwai-Lin
Yeo, Chew-Chieng
Puthucheary, Savithri
Yasin, Rohani
Lim, King-Ting
المصدر
العدد
المجلد 2009، العدد 2009 (31 ديسمبر/كانون الأول 2009)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2009-08-05
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
The emergence of Escherichia coli that produce extended spectrum β-lactamases (ESBLs) and are multidrug resistant (MDR) poses antibiotic management problems.
Forty-seven E.
coli isolates from various public hospitals in Malaysia were studied.
All isolates were sensitive to imipenem whereas 36 were MDR (resistant to 2 or more classes of antibiotics).
PCR detection using gene-specific primers showed that 87.5% of the ESBL-producing E.
coli harbored the blaTEM gene.
Other ESBL-encoding genes detected were blaOXA, blaSHV, and blaCTX-M.
Integron-encoded integrases were detected in 55.3% of isolates, with class 1 integron-encoded intI1 integrase being the majority.
Amplification and sequence analysis of the 5′CS region of the integrons showed known antibiotic resistance-encoding gene cassettes of various sizes that were inserted within the respective integrons.
Conjugation and transformation experiments indicated that some of the antibiotic resistance genes were likely plasmid-encoded and transmissible.
All 47 isolates were subtyped by PFGE and PCR-based fingerprinting using random amplified polymorphic DNA (RAPD), repetitive extragenic palindromes (REPs), and enterobacterial repetitive intergenic consensus (ERIC).
These isolates were very diverse and heterogeneous.
PFGE, ERIC, and REP-PCR methods were more discriminative than RAPD in subtyping the E.
coli isolates.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Lim, King-Ting& Yasin, Rohani& Yeo, Chew-Chieng& Puthucheary, Savithri& Thong, Kwai-Lin. 2009. Characterization of Multidrug Resistant ESBL-Producing Escherichia coli Isolates from Hospitals in Malaysia. BioMed Research International،Vol. 2009, no. 2009, pp.1-10.
https://search.emarefa.net/detail/BIM-988332
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Lim, King-Ting…[et al.]. Characterization of Multidrug Resistant ESBL-Producing Escherichia coli Isolates from Hospitals in Malaysia. BioMed Research International No. 2009 (2009), pp.1-10.
https://search.emarefa.net/detail/BIM-988332
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Lim, King-Ting& Yasin, Rohani& Yeo, Chew-Chieng& Puthucheary, Savithri& Thong, Kwai-Lin. Characterization of Multidrug Resistant ESBL-Producing Escherichia coli Isolates from Hospitals in Malaysia. BioMed Research International. 2009. Vol. 2009, no. 2009, pp.1-10.
https://search.emarefa.net/detail/BIM-988332
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-988332
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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