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Integrative Gene Cloning and Expression System for Streptomyces sp. US 24 and Streptomyces sp. TN 58 Bioactive Molecule Producing Strains
المؤلفون المشاركون
Bejar, Samir
Smaoui, Slim
Karray-Rebai, Ines
Mellouli, Lotfi
Sioud, Samiha
Aigle, Bertrand
المصدر
العدد
المجلد 2009، العدد 2009 (31 ديسمبر/كانون الأول 2009)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2009-05-17
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Streptomyces sp.
US 24 and Streptomyces sp.
TN 58, two strains producing interesting bioactive molecules, were successfully transformed using E.
coli ET12567 (pUZ8002), as a conjugal donor, carrying the integrative plasmid pSET152.
For the Streptomyces sp.
US 24 strain, two copies of this plasmid were tandemly integrated in the chromosome, whereas for Streptomyces sp.
TN 58, the integration was in single copy at the attB site.
Plasmid pSET152 was inherited every time for all analysed Streptomyces sp.
US 24 and Streptomyces sp.
TN 58 exconjugants under nonselective conditions.
The growth, morphological differentiation, and active molecules production of all studied pSET152 integrated exconjugants were identical to those of wild type strains.
Consequently, conjugal transfer using pSET152 integration system is a suitable means of genes transfer and expression for both studied strains.
To validate the above gene transfer system, the glucose isomerase gene (xylA) from Streptomyces sp.
SK was expressed in strain Streptomyces sp.
TN 58.
Obtained results indicated that heterologous glucose isomerase could be expressed and folded effectively.
Glucose isomerase activity of the constructed TN 58 recombinant strain is of about eighteenfold higher than that of the Streptomyces sp.
SK strain.
Such results are certainly of importance due to the potential use of improved strains in biotechnological process for the production of high-fructose syrup from starch.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Sioud, Samiha& Aigle, Bertrand& Karray-Rebai, Ines& Smaoui, Slim& Bejar, Samir& Mellouli, Lotfi. 2009. Integrative Gene Cloning and Expression System for Streptomyces sp. US 24 and Streptomyces sp. TN 58 Bioactive Molecule Producing Strains. BioMed Research International،Vol. 2009, no. 2009, pp.1-10.
https://search.emarefa.net/detail/BIM-988376
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Sioud, Samiha…[et al.]. Integrative Gene Cloning and Expression System for Streptomyces sp. US 24 and Streptomyces sp. TN 58 Bioactive Molecule Producing Strains. BioMed Research International No. 2009 (2009), pp.1-10.
https://search.emarefa.net/detail/BIM-988376
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Sioud, Samiha& Aigle, Bertrand& Karray-Rebai, Ines& Smaoui, Slim& Bejar, Samir& Mellouli, Lotfi. Integrative Gene Cloning and Expression System for Streptomyces sp. US 24 and Streptomyces sp. TN 58 Bioactive Molecule Producing Strains. BioMed Research International. 2009. Vol. 2009, no. 2009, pp.1-10.
https://search.emarefa.net/detail/BIM-988376
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-988376
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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