Production of Polyclonal Antibody against Interleukin-33 and Assessment of Its Distribution in Murine Liver and Lung
المؤلفون المشاركون
Li, Mingcai
Zhou, Yanchun
Chen, Sui
Wu, Yan
Liu, Xiaojin
المصدر
العدد
المجلد 2009، العدد 2009 (31 ديسمبر/كانون الأول 2009)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2009-09-29
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Interleukin (IL)-33 is the latest member of IL-1 cytokine family.
In this study, the cloning, expression, purification, and polyclonal antibody preparation of mouse IL-33 were described.
The coding region of IL-33 mature protein was cloned into the prokaryotic expression vector pET-44.
The recombinant protein, IL-33 containing a hexahistidine tag in the C-terminal, was expressed in Escherichia coli.
The expressed soluble protein was purified by immobilized metal-ion affinity chromatography using Ni2+-nitrilotriacetic acid agarose.
The rabbits were immunized with the purified recombinant protein.
The obtained antiserum was precipitated by saturated ammonium sulfate and then purified by Protein A affinity chromatography.
The sensitivity and specificity of the antibodies were evaluated by enzyme-linked immunosorbent assay and immunohistochemistry.
The high titer (1:32000) polyclonal antibodies with high specificity were obtained by immunizing rabbits with the purified recombinant protein.
Significant expression of IL-33 was seen in mouse liver and lung tissues determined with the anti-IL-33.
The production of the polyclonal antibody against IL-33 provides a good tool for studying the biofunctions of IL-33.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Liu, Xiaojin& Wu, Yan& Li, Mingcai& Chen, Sui& Zhou, Yanchun. 2009. Production of Polyclonal Antibody against Interleukin-33 and Assessment of Its Distribution in Murine Liver and Lung. BioMed Research International،Vol. 2009, no. 2009, pp.1-6.
https://search.emarefa.net/detail/BIM-988434
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhou, Yanchun…[et al.]. Production of Polyclonal Antibody against Interleukin-33 and Assessment of Its Distribution in Murine Liver and Lung. BioMed Research International No. 2009 (2009), pp.1-6.
https://search.emarefa.net/detail/BIM-988434
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Liu, Xiaojin& Wu, Yan& Li, Mingcai& Chen, Sui& Zhou, Yanchun. Production of Polyclonal Antibody against Interleukin-33 and Assessment of Its Distribution in Murine Liver and Lung. BioMed Research International. 2009. Vol. 2009, no. 2009, pp.1-6.
https://search.emarefa.net/detail/BIM-988434
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-988434
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر