In Vitro Ultramorphological Assessment of Apoptosis Induced by Zerumbone on (HeLa )
المؤلفون المشاركون
Mohamed Elhassan Taha, Manal
Abdel Wahab, Siddig Ibrahim
Alzubairi, Adel Sharaf
Mohan, Syam
Abdul, Ahmad Bustamam
المصدر
العدد
المجلد 2009، العدد 2009 (31 ديسمبر/كانون الأول 2009)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2009-02-22
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Zerumbone (ZER), a potential anticancer compound, isolated from the fresh rhizomes of Zingiber zerumbet.
In this investigation, the cytotoxic properties of ZER were evaluated, on cancer cells of human cervix (HeLa), breast and ovary, and normal cells of Chinese Hamster ovary, using MTT assay.
Apoptogenic effects of ZER on HeLa were studied using fluorescence microscopy (AO/PI double staining), scanning and transmission electron microscopy (SEM and TEM), and colorimetric assay of the apoptosis promoter enzyme, caspase-3.
The results of MTT assay showed that ZER has less effect on normal cells compared to cancer cells.
The lowest IC50 of ZER was observed on HeLa cells.
Cytological observations showed nuclear and chromatin condensation, cell shrinkage, multinucleation, abnormalities of mitochondrial cristae, membrane blebbing, holes, cytoplasmic extrusions and formation of apoptotic bodies as confirmed collectively by double staining of AO/PI, SEM and TEM.
Statistical analysis (two-tailed t-test) of differential counting of 200 cells under fluorescence microscope revealed significant difference in apoptotic cells populations between treated and untreated HeLa cells.
In addition, ZER has increased the cellular level of caspase-3 on the treated HeLa cells.
It could be concluded that ZER was able to produce distinctive morphological features of cell death that corresponds to apoptosis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Abdel Wahab, Siddig Ibrahim& Abdul, Ahmad Bustamam& Alzubairi, Adel Sharaf& Mohamed Elhassan Taha, Manal& Mohan, Syam. 2009. In Vitro Ultramorphological Assessment of Apoptosis Induced by Zerumbone on (HeLa ). BioMed Research International،Vol. 2009, no. 2009, pp.1-10.
https://search.emarefa.net/detail/BIM-988444
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Abdel Wahab, Siddig Ibrahim…[et al.]. In Vitro Ultramorphological Assessment of Apoptosis Induced by Zerumbone on (HeLa ). BioMed Research International No. 2009 (2009), pp.1-10.
https://search.emarefa.net/detail/BIM-988444
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Abdel Wahab, Siddig Ibrahim& Abdul, Ahmad Bustamam& Alzubairi, Adel Sharaf& Mohamed Elhassan Taha, Manal& Mohan, Syam. In Vitro Ultramorphological Assessment of Apoptosis Induced by Zerumbone on (HeLa ). BioMed Research International. 2009. Vol. 2009, no. 2009, pp.1-10.
https://search.emarefa.net/detail/BIM-988444
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-988444
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر