A Novel Flow-Perfusion Bioreactor Supports 3D Dynamic Cell Culture

المؤلفون المشاركون

Allori, Alexander C.
Sailon, Alexander M.
Warren, Stephen M.
Reformat, Derek D.
Allen, Robert J.
Davidson, Edward H.

المصدر

BioMed Research International

العدد

المجلد 2009، العدد 2009 (31 ديسمبر/كانون الأول 2009)، ص ص. 1-7، 7ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2009-09-29

دولة النشر

مصر

عدد الصفحات

7

التخصصات الرئيسية

الطب البشري

الملخص EN

Bone engineering requires thicker three-dimensional constructs than the maximum thickness supported by standard cell-culture techniques (2 mm).

A flow-perfusion bioreactor was developed to provide chemotransportation to thick (6 mm) scaffolds.

Methods.

Polyurethane scaffolds, seeded with murine preosteoblasts, were loaded into a novel bioreactor.

Control scaffolds remained in static culture.

Samples were harvested at days 2, 4, 6, and 8 and analyzed for cellular distribution, viability, metabolic activity, and density at the periphery and core.

Results.

By day 8, static scaffolds had a periphery cell density of 67%±5.0%, while in the core it was 0.3%±0.3%.

Flow-perfused scaffolds demonstrated peripheral cell density of 94%±8.3% and core density of 76%±3.1% at day 8.

Conclusions.

Flow perfusion provides chemotransportation to thick scaffolds.

This system may permit high throughput study of 3D tissues in vitro and enable prefabrication of biological constructs large enough to solve clinical problems.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Sailon, Alexander M.& Allori, Alexander C.& Davidson, Edward H.& Reformat, Derek D.& Allen, Robert J.& Warren, Stephen M.. 2009. A Novel Flow-Perfusion Bioreactor Supports 3D Dynamic Cell Culture. BioMed Research International،Vol. 2009, no. 2009, pp.1-7.
https://search.emarefa.net/detail/BIM-988464

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Sailon, Alexander M.…[et al.]. A Novel Flow-Perfusion Bioreactor Supports 3D Dynamic Cell Culture. BioMed Research International No. 2009 (2009), pp.1-7.
https://search.emarefa.net/detail/BIM-988464

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Sailon, Alexander M.& Allori, Alexander C.& Davidson, Edward H.& Reformat, Derek D.& Allen, Robert J.& Warren, Stephen M.. A Novel Flow-Perfusion Bioreactor Supports 3D Dynamic Cell Culture. BioMed Research International. 2009. Vol. 2009, no. 2009, pp.1-7.
https://search.emarefa.net/detail/BIM-988464

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-988464