Association of Interleukin-8 with Cachexia from Patients with Low-Third Gastric Cancer

الملخص EN

Interleukin (IL)-8 has been implicated in the development of cancer cachexia.

The polymorphism of IL-8 gene, which may affect the production level of IL-8, may be associated with cancer cachexia.

Methods.

The serum IL-8 level in our study was examined by radioimmunoassay.

We also analyzed single nucleotide polymorphisms (SNPs) −251 A/T and +781 C/T of IL-8 gene, using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).

Results.

The serum levels of IL-8 were significantly elevated in patients with low-third gastric cancer compared with controls, and were further up-regulated in patients with cachexia than those without (Z=−3.134, P=.002).

A significantly increased frequency of +781 T allele was noted in patients with cachexia (OR=2.247, 95% CI: 1.351–3.737, P=.002).

The +781 TT genotype was observed to be associated with a significantly increased risk of cachexia (OR=3.167, 95% CI: 1.265–7.929, P=.011), and with odds ratio of 3.033 (95% CI: 1.065–8.639, P=.038) for cachexia after adjusting for potential confounding factors.

Meanwhile, haplotype analysis indicated a borderline positive association between T251T781 haplotype and cachexia as compared with the T251C781 haplotype (OR=4.92, 95% CI: 1.00–24.28; ,P=.053).

Conclusions.

IL-8 appears to be associated with cachexia from patients with low-third gastric cancer.