Autocrine, Not Paracrine, Interferon-Gamma Gene Delivery Enhances Ex Vivo Antigen-Specific Cytotoxic T Lymphocyte Stimulation and Killing
المؤلفون المشاركون
Hermonat, Paul L.
Luo, Rong Cheng
Cao, Maohua
Liu, Yong
Zhang, Dazhi
You, Chang Xuan
Shi, Min
المصدر
العدد
المجلد 2010، العدد 2010 (31 ديسمبر/كانون الأول 2009)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2010-05-13
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
The adoptive transfer of antigen-specific cytotoxic T lymphocytes (CTL) shows promise in the treatment of cancer and infectious diseases.
We utilize adeno-associated virus-(AAV-) based antigen gene-loaded dendritic cells (DCs) to stimulate such antigen-specific CTL.
Yet further improvements in CTL stimulation and killing may result by gene delivery of various Th1-response interferons/cytokines, such as interferon γ (IFN-γ), as the delivered gene can continuously produce that interferon.
However which immune cell type should optimally express IFN-γ is unclear as the phenotypes of both DC and T cells are enhanced by it.
Here, we used AAV to compare and contrast IFN-γ gene delivery into DC or T cells, and versus the addition of exogenous IFN-γ, for stimulating carcinoembryonic antigen-(CEA-) specific CTL.
It was found that AAV/IFN-γ delivery into T cells (autocrine) resulted in T cell populations with the highest CD8(+)/CD4(+) ratio, highest IFN-γ(+)/IL-4(+) ratio, highest CD69(+),CD8(+) levels, and lowest CD4(+)/CD25(+) levels, all consistent with the strongest Th1 response.
Most importantly, AAV/IFN-γ transduction of T cells resulted in antigen-specific T cell populations with the highest killing capabilities, 49% above other treatments.
These data strongly suggest that AAV/IFN-γ autocrine gene delivery into T cells is worthy of further study towards maximizing the generation of antigen-specific anticancer CTL killers.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhang, Dazhi& Liu, Yong& Shi, Min& You, Chang Xuan& Cao, Maohua& Luo, Rong Cheng…[et al.]. 2010. Autocrine, Not Paracrine, Interferon-Gamma Gene Delivery Enhances Ex Vivo Antigen-Specific Cytotoxic T Lymphocyte Stimulation and Killing. BioMed Research International،Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-988834
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhang, Dazhi…[et al.]. Autocrine, Not Paracrine, Interferon-Gamma Gene Delivery Enhances Ex Vivo Antigen-Specific Cytotoxic T Lymphocyte Stimulation and Killing. BioMed Research International No. 2010 (Dec. 2010), pp.1-11.
https://search.emarefa.net/detail/BIM-988834
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhang, Dazhi& Liu, Yong& Shi, Min& You, Chang Xuan& Cao, Maohua& Luo, Rong Cheng…[et al.]. Autocrine, Not Paracrine, Interferon-Gamma Gene Delivery Enhances Ex Vivo Antigen-Specific Cytotoxic T Lymphocyte Stimulation and Killing. BioMed Research International. 2010. Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-988834
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-988834
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر