The Vacuolar-Type H+-ATPase in Ovine Rumen Epithelium is Regulated by Metabolic Signals

المؤلفون المشاركون

Kuzinski, Judith
Warnke-Gurgel, Christina
Schweigel, Monika
Zitnan, Rudolf

المصدر

BioMed Research International

العدد

المجلد 2010، العدد 2010 (31 ديسمبر/كانون الأول 2009)، ص ص. 1-12، 12ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2009-12-01

دولة النشر

مصر

عدد الصفحات

12

التخصصات الرئيسية

الطب البشري

الملخص EN

In this study, the effect of metabolic inhibition (MI) by glucose substitution with 2-deoxyglucose (2-DOG) and/or application of antimycin A on ovine rumen epithelial cells (REC) vacuolar-type H+-ATPase (vH+-ATPase) activity was investigated.

Using fluorescent spectroscopy, basal pHi of REC was measured to be 7.3±0.1 in HCO3−-free, glucose-containing NaCl medium.

MI induced a strong pHi reduction (−0.44±0.04 pH units) with a more pronounced effect of 2-DOG compared to antimycin A (−0.30±0.03 versus −0.21±0.03 pH units).

Treatment with foliomycin, a specific vH+-ATPase inhibitor, decreased REC pHi by 0.21±0.05 pH units.

After MI induction, this effect was nearly abolished (−0.03±0.02 pH units).

In addition, membrane-associated localization of vH+-ATPase B subunit disappeared.

Metabolic control of vH+-ATPase involving regulation of its assembly state by elements of the glycolytic pathway could provide a means to adapt REC ATP consumption according to energy availability.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Kuzinski, Judith& Zitnan, Rudolf& Warnke-Gurgel, Christina& Schweigel, Monika. 2009. The Vacuolar-Type H+-ATPase in Ovine Rumen Epithelium is Regulated by Metabolic Signals. BioMed Research International،Vol. 2010, no. 2010, pp.1-12.
https://search.emarefa.net/detail/BIM-988958

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Kuzinski, Judith…[et al.]. The Vacuolar-Type H+-ATPase in Ovine Rumen Epithelium is Regulated by Metabolic Signals. BioMed Research International No. 2010 (Dec. 2010), pp.1-12.
https://search.emarefa.net/detail/BIM-988958

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Kuzinski, Judith& Zitnan, Rudolf& Warnke-Gurgel, Christina& Schweigel, Monika. The Vacuolar-Type H+-ATPase in Ovine Rumen Epithelium is Regulated by Metabolic Signals. BioMed Research International. 2009. Vol. 2010, no. 2010, pp.1-12.
https://search.emarefa.net/detail/BIM-988958

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-988958