Uncovering the Complexity of Transcriptomes with RNA-Seq

المؤلفون المشاركون

Costa, Valerio
De Feis, Italia
Ciccodicola, Alfredo
Angelini, Claudia

المصدر

BioMed Research International

العدد

المجلد 2010، العدد 2010 (31 ديسمبر/كانون الأول 2009)، ص ص. 1-19، 19ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2010-05-16

دولة النشر

مصر

عدد الصفحات

19

التخصصات الرئيسية

الطب البشري

الملخص EN

In recent years, the introduction of massively parallel sequencing platforms for Next Generation Sequencing (NGS) protocols, able to simultaneously sequence hundred thousand DNA fragments, dramatically changed the landscape of the genetics studies.

RNA-Seq for transcriptome studies, Chip-Seq for DNA-proteins interaction, CNV-Seq for large genome nucleotide variations are only some of the intriguing new applications supported by these innovative platforms.

Among them RNA-Seq is perhaps the most complex NGS application.

Expression levels of specific genes, differential splicing, allele-specific expression of transcripts can be accurately determined by RNA-Seq experiments to address many biological-related issues.

All these attributes are not readily achievable from previously widespread hybridization-based or tag sequence-based approaches.

However, the unprecedented level of sensitivity and the large amount of available data produced by NGS platforms provide clear advantages as well as new challenges and issues.

This technology brings the great power to make several new biological observations and discoveries, it also requires a considerable effort in the development of new bioinformatics tools to deal with these massive data files.

The paper aims to give a survey of the RNA-Seq methodology, particularly focusing on the challenges that this application presents both from a biological and a bioinformatics point of view.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Costa, Valerio& Angelini, Claudia& De Feis, Italia& Ciccodicola, Alfredo. 2010. Uncovering the Complexity of Transcriptomes with RNA-Seq. BioMed Research International،Vol. 2010, no. 2010, pp.1-19.
https://search.emarefa.net/detail/BIM-989153

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Costa, Valerio…[et al.]. Uncovering the Complexity of Transcriptomes with RNA-Seq. BioMed Research International No. 2010 (Dec. 2010), pp.1-19.
https://search.emarefa.net/detail/BIM-989153

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Costa, Valerio& Angelini, Claudia& De Feis, Italia& Ciccodicola, Alfredo. Uncovering the Complexity of Transcriptomes with RNA-Seq. BioMed Research International. 2010. Vol. 2010, no. 2010, pp.1-19.
https://search.emarefa.net/detail/BIM-989153

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-989153