![](/images/graphics-bg.png)
Simple and Specific Dual-Wavelength Excitable Dye Staining for Glycoprotein Detection in Polyacrylamide Gels and Its Application in Glycoproteomics
المؤلفون المشاركون
Chiang, Yu-Hsuan
Li, Ding-Tzai
Lin, Tzu-Chun
Wu, Yu-Jen
Chen, Yu-Kuang H.
Hsu, Jue-Liang
Chen, Ching-Chuan
Shi, Fong-Ku
Chen, Kuan-Hung
Lu, Ya-Ting
المصدر
العدد
المجلد 2011، العدد 2011 (31 ديسمبر/كانون الأول 2011)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2011-09-29
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
In this study, a commercially available fluorescent dye, Lissamine rhodamine B sulfonyl hydrazine (LRSH), was designed to specifically stain the glycoproteins in polyacrylamide gels.
Through the periodate/Schiff base mechanism, the fluorescent dye readily attaches to glycoproteins and the fluorescence can be simultaneously observed under either 305 nm or 532 nm excitation therefore, the dye-stained glycoproteins can be detected under a regular UV transilluminator or a more elegant laser-based gel scanner.
The specificity and detection limit were examined using a standard protein mixture in polyacrylamide gels in this study.
The application of this glycoprotein stain dye was further demonstrated using pregnancy urine samples.
The fluorescent spots were further digested in gel and their identities confirmed through LC-MS/MS analysis and database searching.
In addition, the N-glycosylation sites of LRSH-labeled uromodulin were readily mapped via in-gel PNGaseF deglycosylation and LC-MS/MS analysis, which indicated that this fluorescent dye labeling does not interfere with enzymatic deglycosylation.
Hence, the application of this simple and specific dual-wavelength excitable dye staining in current glycoproteome research is promising.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chiang, Yu-Hsuan& Wu, Yu-Jen& Lu, Ya-Ting& Chen, Kuan-Hung& Lin, Tzu-Chun& Chen, Yu-Kuang H.…[et al.]. 2011. Simple and Specific Dual-Wavelength Excitable Dye Staining for Glycoprotein Detection in Polyacrylamide Gels and Its Application in Glycoproteomics. BioMed Research International،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-990281
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chiang, Yu-Hsuan…[et al.]. Simple and Specific Dual-Wavelength Excitable Dye Staining for Glycoprotein Detection in Polyacrylamide Gels and Its Application in Glycoproteomics. BioMed Research International No. 2011 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-990281
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chiang, Yu-Hsuan& Wu, Yu-Jen& Lu, Ya-Ting& Chen, Kuan-Hung& Lin, Tzu-Chun& Chen, Yu-Kuang H.…[et al.]. Simple and Specific Dual-Wavelength Excitable Dye Staining for Glycoprotein Detection in Polyacrylamide Gels and Its Application in Glycoproteomics. BioMed Research International. 2011. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-990281
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-990281
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)