Protein Profiling of Human Nonpigmented Ciliary Epithelium Cell Secretome: The Differentiation Factors Characterization for Retinal Ganglion Cell line
المؤلفون المشاركون
Yang, Ming Hui
Dibas, Adnan
Krishnamoorthy, Raghu R.
Yang, Yuan-Han
Yorio, Thomas
Chen, Wen-Cheng
Chen, Sharon Chia-Ju
Jong, Shiang-Bin
Chu, Pei-Yu
Chung, Tze Wen
Tyan, Yu Chang
المصدر
العدد
المجلد 2011، العدد 2011 (31 ديسمبر/كانون الأول 2011)، ص ص. 1-28، 28ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2011-07-05
دولة النشر
مصر
عدد الصفحات
28
التخصصات الرئيسية
الملخص EN
The purpose of this paper was to characterize proteins secreted from the human nonpigmented ciliary epithelial (HNPE) cells, which have differentiated a rat retinal ganglion cell line, RGC-5.
Undifferentiated RGC-5 cells have been shown to express several marker proteins characteristic of retinal ganglion cells.
However, RGC-5 cells do not respond to N-methyl-D aspartate (NMDA), or glutamate.
HNPE cells have been shown to secrete numbers of neuropeptides or neuroproteins also found in the aqueous humor, many of which have the ability to influence the activity of neuronal cells.
This paper details the profile of HNPE cell-secreted proteins by proteomic approaches.
The experimental results revealed the identification of 132 unique proteins from the HNPE cell-conditioned SF-medium.
The biological functions of a portion of these identified proteins are involved in cell differentiation.
We hypothesized that a differentiation system of HNPE cell-conditioned SF-medium with RGC-5 cells can induce a differentiated phenotype in RGC-5 cells, with functional characteristics that more closely resemble primary cultures of rat retinal ganglion cells.
These proteins may replace harsh chemicals, which are currently used to induce cell differentiation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yang, Ming Hui& Krishnamoorthy, Raghu R.& Jong, Shiang-Bin& Chu, Pei-Yu& Yang, Yuan-Han& Chen, Wen-Cheng…[et al.]. 2011. Protein Profiling of Human Nonpigmented Ciliary Epithelium Cell Secretome: The Differentiation Factors Characterization for Retinal Ganglion Cell line. BioMed Research International،Vol. 2011, no. 2011, pp.1-28.
https://search.emarefa.net/detail/BIM-990367
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yang, Ming Hui…[et al.]. Protein Profiling of Human Nonpigmented Ciliary Epithelium Cell Secretome: The Differentiation Factors Characterization for Retinal Ganglion Cell line. BioMed Research International No. 2011 (2011), pp.1-28.
https://search.emarefa.net/detail/BIM-990367
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yang, Ming Hui& Krishnamoorthy, Raghu R.& Jong, Shiang-Bin& Chu, Pei-Yu& Yang, Yuan-Han& Chen, Wen-Cheng…[et al.]. Protein Profiling of Human Nonpigmented Ciliary Epithelium Cell Secretome: The Differentiation Factors Characterization for Retinal Ganglion Cell line. BioMed Research International. 2011. Vol. 2011, no. 2011, pp.1-28.
https://search.emarefa.net/detail/BIM-990367
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-990367
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر