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Treatment of Mycoplasma Contamination in Cell Cultures with Plasmocin
المؤلفون المشاركون
Uphoff, Cord C.
Denkmann, Sabine-A.
Drexler, Hans G.
المصدر
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-10-02
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
A high percentage of cell lines are chronically infected with various mycoplasma species.
The addition of antibiotics that are particularly effective against these contaminants to the culture medium during a limited period of time is a simple, inexpensive, and very practical approach for decontaminating cell cultures.
Here, we examined the effectiveness of the new antimycoplasma compound Plasmocin that has been employed routinely to cleanse chronically infected cell lines.
In a first round of treatment 45 out of 58 (78%) mycoplasma-positive cell lines could be cured.
In a second attempt using back-up cryopreserved original cells, four additional cell lines were cured; thus, the overall cure rate was 84%.
Even if the mycoplasma contamination was not eradicated by Plasmocin, the parallel treatment with several other antibiotics (Baytril, BM-Cyclin, Ciprobay, MRA, or MycoZap) led to the cure of all 58 cell lines.
The successful decontamination was permanent as mycoplasmas were no longer detected at day +14 posttreatment and at later time points as examined by PCR which is the most sensitive and specific mycoplasma detection method.
Collectively, our results highlight certain antibiotics as effective antimycoplasma reagents and support the therapeutic rationale for their use in the eradication of this notorious cell culture contaminant.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Uphoff, Cord C.& Denkmann, Sabine-A.& Drexler, Hans G.. 2012. Treatment of Mycoplasma Contamination in Cell Cultures with Plasmocin. BioMed Research International،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-991610
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Uphoff, Cord C.…[et al.]. Treatment of Mycoplasma Contamination in Cell Cultures with Plasmocin. BioMed Research International No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-991610
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Uphoff, Cord C.& Denkmann, Sabine-A.& Drexler, Hans G.. Treatment of Mycoplasma Contamination in Cell Cultures with Plasmocin. BioMed Research International. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-991610
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-991610
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
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