Multiple Signaling Pathways Are Involved in the Interleukine-4 Regulated Expression of DC-SIGN in THP-1 Cell Line
المؤلفون المشاركون
Fang, Meixin
Jin, Changzhong
Wu, Nanping
Li, Jie
Wu, Lijuan
Cheng, Linfang
المصدر
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-03-12
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Dendritic cell-specific intercellular adhesion molecule-3 grabbing nonintegrin (DC-SIGN) is an important pattern recognition receptor on dendritic cells (DCs), and its expression shows significant cytological and histological specificity, being interleukine-4 (IL-4) dependent.
The signaling pathways through which IL-4 regulates expression of DC-SIGN are still unclear.
We used phorbol 12-myristate 13-acetate- (PMA-) differentiated THP-1 cells as the in vitro model of monocyte/macrophage cells to study the signaling pathways involved in IL-4-regulated expression of DC-SIGN.
We found that a high expression of DC-SIGN could be induced by IL-4 at the levels of mRNA and cell surface protein.
Upregulated expression of DC-SIGN was almost completely blocked by the specific inhibitor of ERK pathway, and partly reduced by the specific inhibitors of JAK-STAT and NF-κB pathways.
The activation of the three signaling pathways was directly confirmed by testing the phosphorylation of protein kinase within the cytoplasm and nucleus over time.
The analysis of cis-acting elements of DC-SIGN promoter showed that the activity of DC-SIGN promoter without Ets-1 transcription factors binding site almost completely disappeared.
Our results demonstrated that multiple signaling pathways are involved in IL-4 induced high expression of DC-SIGN on THP-1 cells, in which ERK pathway is the main signaling pathway and mediated by the Ets-1 transcription factors binding site.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Jin, Changzhong& Wu, Lijuan& Li, Jie& Fang, Meixin& Cheng, Linfang& Wu, Nanping. 2012. Multiple Signaling Pathways Are Involved in the Interleukine-4 Regulated Expression of DC-SIGN in THP-1 Cell Line. BioMed Research International،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-991653
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Jin, Changzhong…[et al.]. Multiple Signaling Pathways Are Involved in the Interleukine-4 Regulated Expression of DC-SIGN in THP-1 Cell Line. BioMed Research International No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-991653
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Jin, Changzhong& Wu, Lijuan& Li, Jie& Fang, Meixin& Cheng, Linfang& Wu, Nanping. Multiple Signaling Pathways Are Involved in the Interleukine-4 Regulated Expression of DC-SIGN in THP-1 Cell Line. BioMed Research International. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-991653
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-991653
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر