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Cytotoxicity, Antiproliferative Effects, and Apoptosis Induction of Methanolic Extract of Cynometra cauliflora Linn. Whole Fruit on Human Promyelocytic Leukemia HL-60 Cells
المؤلفون المشاركون
Tajudin, T-Johari S. A.
Mat, Nashriyah
Siti-Aishah, Abu Bakar
Yusran, A. Aziz M.
Alwi, Afnani
Ali, Abdul Manaf
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-11-04
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Methanolic extract of Cynometra cauliflora whole fruit was assayed for cytotoxicity against the human promyelocytic leukemia HL-60 and the normal mouse fibroblast NIH/3T3 cell lines by using the MTT assay.
The CD50 of the extract for 72 hours was 0.9 μg/mL whereas the value for the cytotoxic drug vincristine was 0.2 μg/mL.
The viability of the NIH/3T3 cells was at 80.0% when treated at 15.0 μg/mL.
The extract inhibited HL-60 cell proliferation with dose dependence.
AO/PI staining of HL-60 cells treated with the extract revealed that majority of cells were in the apoptotic cell death mode.
Flow cytometry analysis of HL-60 cells treated at CD50 of the extract showed that the early apoptotic cells were 31.0, 26.3 and 19.9% at 24, 48, and 72 hours treatment, respectively.
The percentage of late apoptotic cells was increased from 62.0 at 24 hours to 64.1 and 70.2 at 48 and 72 hours, respectively.
Meanwhile, percent of necrotic cells were 4.9, 6.6, and 8.5 at 24, 48, and 72 hours, respectively.
This study has shown that the methanolic extract of C.
cauliflora whole fruit was cytotoxic towards HL-60 cells and induced the cells into apoptotic cell death mode, but less cytotoxic towards NIH/3T3 cells.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Tajudin, T-Johari S. A.& Mat, Nashriyah& Siti-Aishah, Abu Bakar& Yusran, A. Aziz M.& Alwi, Afnani& Ali, Abdul Manaf. 2012. Cytotoxicity, Antiproliferative Effects, and Apoptosis Induction of Methanolic Extract of Cynometra cauliflora Linn. Whole Fruit on Human Promyelocytic Leukemia HL-60 Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-992014
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Tajudin, T-Johari S. A.…[et al.]. Cytotoxicity, Antiproliferative Effects, and Apoptosis Induction of Methanolic Extract of Cynometra cauliflora Linn. Whole Fruit on Human Promyelocytic Leukemia HL-60 Cells. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-992014
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Tajudin, T-Johari S. A.& Mat, Nashriyah& Siti-Aishah, Abu Bakar& Yusran, A. Aziz M.& Alwi, Afnani& Ali, Abdul Manaf. Cytotoxicity, Antiproliferative Effects, and Apoptosis Induction of Methanolic Extract of Cynometra cauliflora Linn. Whole Fruit on Human Promyelocytic Leukemia HL-60 Cells. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-992014
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-992014
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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