L(+) and D(-) Lactate Are Increased in Plasma and Urine Samples of Type 2 Diabetes as Measured by a Simultaneous Quantification of L(+) and D(-) Lactate by Reversed-Phase Liquid Chromatography Tandem Mass Spectrometry
المؤلفون المشاركون
Waarenburg, Marjo P. H. van de
Hanssen, Nordin M. J.
Stehouwer, Coen D. A.
Schalkwijk, Casper G.
Scheijen, Jean L. J. M.
Jonkers, Daisy M. A. E.
المصدر
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2011)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-03-08
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Plasma and urinary levels of D-lactate have been linked to the presence of diabetes.
Previously developed techniques have shown several limitations to further evaluate D-lactate as a biomarker for this condition.
Methods.
D- and L-lactate were quantified using ultraperformance liquid chromatography tandem mass spectrometry with labelled internal standard.
Samples were derivatized with diacetyl-L-tartaric anhydride and separated on a C18-reversed phase column.
D- and L-lactate were analysed in plasma and urine of controls, patients with inflammatory bowel disease (IBD), and patients with type 2 diabetes (T2DM).
Results.
Quantitative analysis of D- and L-lactate was achieved successfully.
Calibration curves were linear (r2>0.99) over the physiological and pathophysiological ranges.
Recoveries for urine and plasma were between 96% and 113%.
Inter- and intra-assay variations were between 2% and 9%.
The limits of detection of D-lactate and L-lactate in plasma were 0.7 μmol/L and 0.2 μmol/L, respectively.
The limits of detection of D-lactate and L-lactate in urine were 8.1 nmol/mmol creatinine and 4.4 nmol/mmol creatinine, respectively.
Plasma and urinary levels of D- and L-lactate were increased in patients with IBD and T2DM as compared with controls.
Conclusion.
The presented method proved to be suitable for the quantification of D- and L-lactate and opens the possibility to explore the use of D-lactate as a biomarker.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Scheijen, Jean L. J. M.& Hanssen, Nordin M. J.& Waarenburg, Marjo P. H. van de& Jonkers, Daisy M. A. E.& Stehouwer, Coen D. A.& Schalkwijk, Casper G.. 2012. L(+) and D(-) Lactate Are Increased in Plasma and Urine Samples of Type 2 Diabetes as Measured by a Simultaneous Quantification of L(+) and D(-) Lactate by Reversed-Phase Liquid Chromatography Tandem Mass Spectrometry. Journal of Diabetes Research،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-993943
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Scheijen, Jean L. J. M.…[et al.]. L(+) and D(-) Lactate Are Increased in Plasma and Urine Samples of Type 2 Diabetes as Measured by a Simultaneous Quantification of L(+) and D(-) Lactate by Reversed-Phase Liquid Chromatography Tandem Mass Spectrometry. Journal of Diabetes Research Vol. 2012, no. 2012 (2011), pp.1-10.
https://search.emarefa.net/detail/BIM-993943
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Scheijen, Jean L. J. M.& Hanssen, Nordin M. J.& Waarenburg, Marjo P. H. van de& Jonkers, Daisy M. A. E.& Stehouwer, Coen D. A.& Schalkwijk, Casper G.. L(+) and D(-) Lactate Are Increased in Plasma and Urine Samples of Type 2 Diabetes as Measured by a Simultaneous Quantification of L(+) and D(-) Lactate by Reversed-Phase Liquid Chromatography Tandem Mass Spectrometry. Journal of Diabetes Research. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-993943
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-993943
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر