KU-32, a Novel Drug for Diabetic Neuropathy, Is Safe for Human Islets and Improves In Vitro Insulin Secretion and Viability
المؤلفون المشاركون
Rawal, Sonia
Williams, S. Janette
Novikova, Lesya
Ramachandran, Karthik
Dobrowsky, Rick
Stehno-Bittel, Lisa
Farmer, Kevin
Blagg, Brian S. J.
المصدر
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2011)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-11-01
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
KU-32 is a novel, novobiocin-based Hsp90 inhibitor that protects against neuronal glucotoxicity and reverses multiple clinical indices of diabetic peripheral neuropathy in a rodent model.
However, any drug with potential for treating diabetic complications must also have no adverse effects on the function of pancreatic islets.
Thus, the goal of the current study was to assess the effect of KU-32 on the in vitro viability and function of human islets.
Treating human islets with KU-32 for 24 hours showed no toxicity as assessed using the alamarBlue assay.
Confocal microscopy confirmed that with a minimum of 2-day exposure, KU-32 improved cellular viability by blocking apoptosis.
Functionally, isolated human islets released more glucose-stimulated insulin when preincubated in KU-32.
However, diabetic BKS-db/db mice, a model for type 2 diabetes, administered KU-32 for 10 weeks did not show any significant changes in blood glucose and insulin levels, despite having greater insulin staining/beta cell in the pancreas compared to untreated BKS db/db mice.
In summary, KU-32 did not harm isolated human islets and may even be protective.
However, the effect does not appear significant enough to alter the in vivo metabolic parameters of diabetic mice.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Farmer, Kevin& Williams, S. Janette& Novikova, Lesya& Ramachandran, Karthik& Rawal, Sonia& Blagg, Brian S. J.…[et al.]. 2012. KU-32, a Novel Drug for Diabetic Neuropathy, Is Safe for Human Islets and Improves In Vitro Insulin Secretion and Viability. Journal of Diabetes Research،Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-994046
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Farmer, Kevin…[et al.]. KU-32, a Novel Drug for Diabetic Neuropathy, Is Safe for Human Islets and Improves In Vitro Insulin Secretion and Viability. Journal of Diabetes Research Vol. 2012, no. 2012 (2011), pp.1-11.
https://search.emarefa.net/detail/BIM-994046
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Farmer, Kevin& Williams, S. Janette& Novikova, Lesya& Ramachandran, Karthik& Rawal, Sonia& Blagg, Brian S. J.…[et al.]. KU-32, a Novel Drug for Diabetic Neuropathy, Is Safe for Human Islets and Improves In Vitro Insulin Secretion and Viability. Journal of Diabetes Research. 2012. Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-994046
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-994046
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر