Oxidative Metabolism Genes Are Not Responsive to Oxidative Stress in Rodent Beta Cell Lines

المؤلفون المشاركون

Morrison, Faer
Harries, Lorna W.
Johnstone, Karen
Locke, Jonathan
Murray, Anna

المصدر

Journal of Diabetes Research

العدد

المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2011)، ص ص. 1-5، 5ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2011-12-15

دولة النشر

مصر

عدد الصفحات

5

التخصصات الرئيسية

الأمراض
الطب البشري

الملخص EN

Altered expression of oxidative metabolism genes has been described in the skeletal muscle of individuals with type 2 diabetes.

Pancreatic beta cells contain low levels of antioxidant enzymes and are particularly susceptible to oxidative stress.

In this study, we explored the effect of hyperglycemia-induced oxidative stress on a panel of oxidative metabolism genes in a rodent beta cell line.

We exposed INS-1 rodent beta cells to low (5.6 mmol/L), ambient (11 mmol/L), and high (28 mmol/L) glucose conditions for 48 hours.

Increases in oxidative stress were measured using the fluorescent probe dihydrorhodamine 123.

We then measured the expression levels of a panel of 90 oxidative metabolism genes by real-time PCR.

Elevated reactive oxygen species (ROS) production was evident in INS-1 cells after 48 hours (P<0.05).

TLDA analysis revealed a significant (P<0.05) upregulation of 16 of the 90 genes under hyperglycemic conditions, although these expression differences did not reflect differences in ROS.

We conclude that although altered glycemia may influence the expression of some oxidative metabolism genes, this effect is probably not mediated by increased ROS production.

The alterations to the expression of oxidative metabolism genes previously observed in human diabetic skeletal muscle do not appear to be mirrored in rodent pancreatic beta cells.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Morrison, Faer& Johnstone, Karen& Murray, Anna& Locke, Jonathan& Harries, Lorna W.. 2011. Oxidative Metabolism Genes Are Not Responsive to Oxidative Stress in Rodent Beta Cell Lines. Journal of Diabetes Research،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-994089

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Morrison, Faer…[et al.]. Oxidative Metabolism Genes Are Not Responsive to Oxidative Stress in Rodent Beta Cell Lines. Journal of Diabetes Research Vol. 2012, no. 2012 (2011), pp.1-5.
https://search.emarefa.net/detail/BIM-994089

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Morrison, Faer& Johnstone, Karen& Murray, Anna& Locke, Jonathan& Harries, Lorna W.. Oxidative Metabolism Genes Are Not Responsive to Oxidative Stress in Rodent Beta Cell Lines. Journal of Diabetes Research. 2011. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-994089

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-994089