Epithelial-to-Mesenchymal Transition in Pancreatic Ductal Adenocarcinoma and Pancreatic Tumor Cell Lines: The Role of Neutrophils and Neutrophil-Derived Elastase
المؤلفون المشاركون
Giese, Thomas
Große-Steffen, Thomas
Longerich, Thomas
Hänsch, Gertrud Maria
Giese, Nathalia
Gaida, Matthias M.
Schirmacher, Peter
المصدر
Journal of Immunology Research
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2011)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-11-20
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Pancreatic ductal adenocarcinoma (PDAC) is frequently associated with fibrosis and a prominent inflammatory infiltrate in the desmoplastic stroma.
Moreover, in PDAC, an epithelial-to-mesenchymal transition (EMT) is observed.
To explore a possible connection between the infiltrating cells, particularly the polymorphonuclear neutrophils (PMN) and the tumor cell transition, biopsies of patients with PDAC (n=115) were analysed with regard to PMN infiltration and nuclear expression of β-catenin and of ZEB1, well-established indicators of EMT.
In biopsies with a dense PMN infiltrate, a nuclear accumulation of β-catenin and of ZEB1 was observed.
To address the question whether PMN could induce EMT, they were isolated from healthy donors and were cocultivated with pancreatic tumor cells grown as monolayers.
Rapid dyshesion of the tumor cells was seen, most likely due to an elastase-mediated degradation of E-cadherin.
In parallel, the transcription factor TWIST was upregulated, β-catenin translocated into the nucleus, ZEB1 appeared in the nucleus, and keratins were downregulated.
EMT was also induced when the tumor cells were grown under conditions preventing attachment to the culture plates.
Here, also in the absence of elastase, E-cadherin was downmodulated.
PMN as well as prevention of adhesion induced EMT also in liver cancer cell line.
In conclusion, PMN via elastase induce EMT in vitro, most likely due to the loss of cell-to-cell contact.
Because in pancreatic cancers the transition to a mesenchymal phenotype coincides with the PMN infiltrate, a contribution of the inflammatory response to the induction of EMT and—by implication—to tumor progression is possible.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Große-Steffen, Thomas& Giese, Thomas& Giese, Nathalia& Longerich, Thomas& Schirmacher, Peter& Hänsch, Gertrud Maria…[et al.]. 2012. Epithelial-to-Mesenchymal Transition in Pancreatic Ductal Adenocarcinoma and Pancreatic Tumor Cell Lines: The Role of Neutrophils and Neutrophil-Derived Elastase. Journal of Immunology Research،Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-996797
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Große-Steffen, Thomas…[et al.]. Epithelial-to-Mesenchymal Transition in Pancreatic Ductal Adenocarcinoma and Pancreatic Tumor Cell Lines: The Role of Neutrophils and Neutrophil-Derived Elastase. Journal of Immunology Research Vol. 2012, no. 2012 (2011), pp.1-12.
https://search.emarefa.net/detail/BIM-996797
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Große-Steffen, Thomas& Giese, Thomas& Giese, Nathalia& Longerich, Thomas& Schirmacher, Peter& Hänsch, Gertrud Maria…[et al.]. Epithelial-to-Mesenchymal Transition in Pancreatic Ductal Adenocarcinoma and Pancreatic Tumor Cell Lines: The Role of Neutrophils and Neutrophil-Derived Elastase. Journal of Immunology Research. 2012. Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-996797
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-996797
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر