Optimization of poly-β-hydroxybutyrate (phb) production by an egyption strain of rhizobium fabae f44 using response surface methodology
العناوين الأخرى
تعظيم إنتاجية البولي بيتا هيدروكسي بيوتيرات بواسطة سلالة مصرية من Rhizobium fabae باستخدام منهجية سطح الاستجابة
المؤلفون المشاركون
Radwan, Wafa Hamdi
Abu Husayn, Samah Hashim
al-Haddad, Muhammad al-Sayyid
Abd al-Maqsud, Nasif Mahmud
Salim, Shawqi Mahmud
المصدر
Arab Universities Journal of Agricultural Sciences
العدد
المجلد 27، العدد 2 (31 مايو/أيار 2019)، ص ص. 1383-1397، 15ص.
الناشر
تاريخ النشر
2019-05-31
دولة النشر
مصر
عدد الصفحات
15
التخصصات الرئيسية
الموضوعات
الملخص EN
The present work is aimed to isolate, purify, identify a newly isolate Rhizobium fabae from different regions in Egypt then, produce and optimize Poly-β-Hydroxybutyrate polymer (PHB) production by the selected isolate Rhizobium fabae F44 using a statistical approach of response surface methodology (RSM).
Rhizobium fabae F44 isolate was picked up from Sharkia Governorate in Egypt and then identified by the 16SrRNA gene as Rhizobium fabae.
Eleven different variables which affect the production of PHB polymer were screened by Plackett–Burman statistical design approach.
Maximization of PHB production was adjusted by the terms of The Face Central Composite Design (FCCD) of RSM and assessed at three coded levels (–1, 0, +1).
One way Anova was used to statistically analyze all obtained results in relation to post hoc multiple comparison analysis performed by Tukey's HSD.
Appulses of nutritional and physical factors (two carbon sources, two nitrogen sources, mineral salts, pH, temperature, incubation time, inoculum size and agitation rate) were studied using Plackett-Burman design method.
58 isolates were obtained from the different 9 governorates with 100 % infection plant technique.
The 16SrRNA showed a similarity of 99.56 (% ) to Rhizobium fabae F44.
Out of all tested significant media components, sucrose, yeast extract, agitation rate had the highest significant effects on the response as for PHB production, with confidence level > 98% and were further optimized using FCCD.
Predicted Maximum PHB production was observed as (78.51% ) which in near the mid-point (0) values (concentrations) of sucrose which reached (25 g/l) and yeast extract of (0.5 g/l) for 48 hrs.
at 150 rpm agitation rate.
The observed experimental value reached 87.5u/ml was very close result to the predicted one validating the model.
So, Response Surface Methodology is an effective statistical approach which can substitute the use of one variable at a time approach due to its adequacy and efficiency to optimize PHB production by Rhizobium fabae F44.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Radwan, Wafa Hamdi& Abu Husayn, Samah Hashim& al-Haddad, Muhammad al-Sayyid& Abd al-Maqsud, Nasif Mahmud& Salim, Shawqi Mahmud. 2019. Optimization of poly-β-hydroxybutyrate (phb) production by an egyption strain of rhizobium fabae f44 using response surface methodology. Arab Universities Journal of Agricultural Sciences،Vol. 27, no. 2, pp.1383-1397.
https://search.emarefa.net/detail/BIM-999757
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Radwan, Wafa Hamdi…[et al.]. Optimization of poly-β-hydroxybutyrate (phb) production by an egyption strain of rhizobium fabae f44 using response surface methodology. Arab Universities Journal of Agricultural Sciences Vol. 27, no. 2 (2019), pp.1383-1397.
https://search.emarefa.net/detail/BIM-999757
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Radwan, Wafa Hamdi& Abu Husayn, Samah Hashim& al-Haddad, Muhammad al-Sayyid& Abd al-Maqsud, Nasif Mahmud& Salim, Shawqi Mahmud. Optimization of poly-β-hydroxybutyrate (phb) production by an egyption strain of rhizobium fabae f44 using response surface methodology. Arab Universities Journal of Agricultural Sciences. 2019. Vol. 27, no. 2, pp.1383-1397.
https://search.emarefa.net/detail/BIM-999757
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
-
رقم السجل
BIM-999757
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر