Isolation and identification of halophilic bacteria producing exopolysaccharides from whey and milk permeate
العناوين الأخرى
عزل و تعريف البكتريا المحبة للملوحة المنتجة للسكريدات العديدة الخارجية من الشرش و راشح اللبن
المؤلفون المشاركون
al-Nawawi, Muhammad Abd al-Raziq
Hijazi, Amal Abd Allah
al-Samraji, Yahya Abd al-Azim
Ali, Ali Abd al-Aziz
المصدر
Arab Universities Journal of Agricultural Sciences
العدد
المجلد 27، العدد 2 (31 مايو/أيار 2019)، ص ص. 1491-1501، 11ص.
الناشر
تاريخ النشر
2019-05-31
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الموضوعات
الملخص EN
The aim of this research was to utilization of the salt whey and milk permeate to produce exopolysaccharides (EPSs).
Where cheese whey is simultaneously an effluent with nutritional value and a strong organic and saline content.
However, it is drained in the sewers, the EPSs are highly heterogeneous polymers produced by different species of bacteria and have recently been attracting considerable attention from biotechnologists because of their potential applications in many fields.
Thus, we have isolated some halophilic bacteria that showed the ability to produce EPS from whey and milk permeate.
A total of 46 strains of moderately halophilic bacteria were isolated from two types of samples.
The first type was dairy samples (Baramily cheese whey and mish cheese), while the second type was water samples (salty lake water and its sediments) of Wadi El-Natrun Valley (lakes Hamra, UmRisha, and Baida), Beheira Governorate, Egypt.
From isolated strains there are twelve strains were having the ability to produce exopolysaccharides but only seven strains can produce EPS from whey and milk permeate.
The growth conditions i.
e.
concentrations of NaCl, pH value and different incubation temperature, of isolates were determined.
The effect of these conditions on the production of EPS was investigated.
The obtained results indicated that the optimum conditions for the production of EPS by these strains were 10 % NaCl, pH 7 and the optimum incubation temperature was 37°C.
Three strains showed the highest production of exopolysaccharides.
These strains were identified using two methods the first method was biology system and the second one was 16S rRNA sequence analysis method.
It could be identified as Alteribacillus bidgolensis and Bacillus licheniformis.
Alteribacillus bidgolensis (strain P4B) produced the highest amount of EPS (52 g/L) from whey followed by Bacillus licheniformis (DSM 13) (42 g/L), while the highest amount of EPS produced from milk permeate was (43 g/L) by Alteribacillus bidgolensis (strain P4B) followed by Bacillus licheniformis (DSM 13) (36 g/L).
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Hijazi, Amal Abd Allah& al-Nawawi, Muhammad Abd al-Raziq& Ali, Ali Abd al-Aziz& al-Samraji, Yahya Abd al-Azim. 2019. Isolation and identification of halophilic bacteria producing exopolysaccharides from whey and milk permeate. Arab Universities Journal of Agricultural Sciences،Vol. 27, no. 2, pp.1491-1501.
https://search.emarefa.net/detail/BIM-999764
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Hijazi, Amal Abd Allah…[et al.]. Isolation and identification of halophilic bacteria producing exopolysaccharides from whey and milk permeate. Arab Universities Journal of Agricultural Sciences Vol. 27, no. 2 (2019), pp.1491-1501.
https://search.emarefa.net/detail/BIM-999764
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Hijazi, Amal Abd Allah& al-Nawawi, Muhammad Abd al-Raziq& Ali, Ali Abd al-Aziz& al-Samraji, Yahya Abd al-Azim. Isolation and identification of halophilic bacteria producing exopolysaccharides from whey and milk permeate. Arab Universities Journal of Agricultural Sciences. 2019. Vol. 27, no. 2, pp.1491-1501.
https://search.emarefa.net/detail/BIM-999764
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
-
رقم السجل
BIM-999764
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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