Isolation and genotyping study of Clostridium Perfringens from broiler farms infected with necrotic enteritis in Sulaimania province
Other Title(s)
عزل و دراسة التنميط الجيني لجرثومة المطثيات البرفنجية المعزولة من فروج اللحم المصاب بخمج الأمعاء التنخري في محافظة السليمانية
Joint Authors
Abd al-Rahman, Nawzad Rashid
Amin, Suzan Qadir
Aziz, Sadat Abd Allah
Source
al-Anbar Journal of Veterinary Sciences
Issue
Vol. 12, Issue 2 (31 Dec. 2019), pp.52-69, 18 p.
Publisher
Publication Date
2019-12-31
Country of Publication
Iraq
No. of Pages
18
Main Subjects
Topics
- Intestines
- Inflammation
- Broilers(Chickens)
- Clostridial enteritis
- Clostridium perfringens
- Nucleotide sequence
Abstract EN
The study was conducted to isolate and toxinotype the suspected cases of Clostridium perfringens infections of broiler farms in Sulaimania province.
A total of 108 samples were collected from intestinal contents, mucosal scraping, and hemorrhagic lymphoid nodules from suspected cases of necrotic enteritis in broilers.
The result of isolated and identified bacteria were revealed that 63 (58% ) out of 108 samples were positive for C.
perfringens.
The results revealed that the isolates were only positive for alpha and beta2 toxin genes.
Phylogenetic and DNA sequence analysis of cpa and cpb2 gene showed that cpa genes were highly identical to isolates from broiler in Iran, poultry stool and broiler in Brazil, and blue calves in Belgium.
While cpb2 gene is closely related to the isolates of broiler in Iran, India and isolates of goat in Pakistan.
The results indicated that the causative agent of necrotic enteritis in broiler farms in the region was mainly due to C.
perfringens type A infection
American Psychological Association (APA)
Amin, Suzan Qadir& Abd al-Rahman, Nawzad Rashid& Aziz, Sadat Abd Allah. 2019. Isolation and genotyping study of Clostridium Perfringens from broiler farms infected with necrotic enteritis in Sulaimania province. al-Anbar Journal of Veterinary Sciences،Vol. 12, no. 2, pp.52-69.
https://search.emarefa.net/detail/BIM-1080521
Modern Language Association (MLA)
Amin, Suzan Qadir…[et al.]. Isolation and genotyping study of Clostridium Perfringens from broiler farms infected with necrotic enteritis in Sulaimania province. al-Anbar Journal of Veterinary Sciences Vol. 12, no. 2 (2019), pp.52-69.
https://search.emarefa.net/detail/BIM-1080521
American Medical Association (AMA)
Amin, Suzan Qadir& Abd al-Rahman, Nawzad Rashid& Aziz, Sadat Abd Allah. Isolation and genotyping study of Clostridium Perfringens from broiler farms infected with necrotic enteritis in Sulaimania province. al-Anbar Journal of Veterinary Sciences. 2019. Vol. 12, no. 2, pp.52-69.
https://search.emarefa.net/detail/BIM-1080521
Data Type
Journal Articles
Language
English
Notes
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Record ID
BIM-1080521