استخلاص و تنقية و توصيف أنزيم الألفا-اميليز المستخلص من حبوب الذرة البيضاء المنبتة

Abstract EN

The study included germination of sorghum cereal and alpha- amylase was followed up during the germination stages, the results showed increase in the activity of amylase in five day from germination 1600 U/ml.

after that was selected of the best methods to extract the enzyme among fore methods.

The purification stages were done by two steps included precipitation by ammonium sulphate at ( 30-70 ) % saturation and gel filtration on sephadex G100.

The final purification folds and the yield of the enzyme were 19.31.

times and 14.60%, respectively.

The purified enzyme showed following characteristics : The optimum pH of the enzyme activity was 6.

The enzyme was most stable at pH ( 4.5 - 6 ).

The optimum temperature for its activity was 65 c.

The enzyme retained its original activity when incubates at ( 30-70 ) c for 20 minutes.