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Some studies on rabbits experimentally infected by e.coli o157 : h7 isolated from raw milk
Other Title(s)
بعض الدراسات علي الأرانب المصابة تجريبيا ببكتريا الايشريشيا القولونية O157 : H7 المعزولة هي اللبن الخام
Joint Authors
al-Jidawi, Atiyyah Abd Allah
Abd al-Ghaffar, Abir al-Rifai
Rashid, Nismah
Shukri, Iman
al-Khawajah, Iman
Ibrahim, Raniya Ibrahim Muhammad
Source
Assiut Veterinary Medical Journal
Issue
Vol. 62, Issue 148 (31 Jan. 2016), pp.69-76, 8 p.
Publisher
Assiut University Faculty of Veterinary Medicine
Publication Date
2016-01-31
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Topics
Abstract EN
Escherichia coli O 157:H7 causes outbreaks of diarrhea and hemolytic uremic syndrome which can cause acute kidney failure in human.
Cattle are identified as the primary source of infection.
In this study, the isolation of Escherichia coli O157: H7 was occurred through out of 100 milk samples from different localities in Egypt.
The positive E.coli isolated was 45 from samples then serological identification was done to identify the serotypes of E.coli which revealed that only one sample donated to E.coli O157: H7.This only positive sample used for infection of rabbit models, which was used to evaluate the pathogenesis of E.coli O157: H7.The 10 rabbits were divided into: control group (3 rabbits) and experimental group (7 rabbits).
Rabbits were inoculated by feeding tube with 109 cfu of E.coliO157: H7.Fecal samples were collected from animals on days 2, 4, 6 post inoculation to determine the shedding of the pathogen.
On day 13 after inoculation, intestine, kidney and liver were collected from slaughtered animals for histopathologic examination.
The intestine showed necrosis and desquamation of epithelial lining with hyperplasia and neutrophilic infiltration.
Kidneys showed microthrombosis in glomeruli and hyaline casts in the lumen of renal tubules.
Liver showed severe congestion with sharp vacuoles in the cytoplasm of hepatocytes giving the cell the signet ring appearance
American Psychological Association (APA)
al-Jidawi, Atiyyah Abd Allah& Abd al-Ghaffar, Abir al-Rifai& Rashid, Nismah& Ibrahim, Raniya Ibrahim Muhammad& Shukri, Iman& al-Khawajah, Iman. 2016. Some studies on rabbits experimentally infected by e.coli o157 : h7 isolated from raw milk. Assiut Veterinary Medical Journal،Vol. 62, no. 148, pp.69-76.
https://search.emarefa.net/detail/BIM-679740
Modern Language Association (MLA)
al-Jidawi, Atiyyah Abd Allah…[et al.]. Some studies on rabbits experimentally infected by e.coli o157 : h7 isolated from raw milk. Assiut Veterinary Medical Journal Vol. 62, no. 148 (Jan. 2016), pp.69-76.
https://search.emarefa.net/detail/BIM-679740
American Medical Association (AMA)
al-Jidawi, Atiyyah Abd Allah& Abd al-Ghaffar, Abir al-Rifai& Rashid, Nismah& Ibrahim, Raniya Ibrahim Muhammad& Shukri, Iman& al-Khawajah, Iman. Some studies on rabbits experimentally infected by e.coli o157 : h7 isolated from raw milk. Assiut Veterinary Medical Journal. 2016. Vol. 62, no. 148, pp.69-76.
https://search.emarefa.net/detail/BIM-679740
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 75-76
Record ID
BIM-679740