الكشف عن الفطر Polymyxa betae Keskin باستخدام الماسح الإلكتروني و عن الفيروسات المنقولة بالتربة التي تصيب الشوندر السكري البنجر في سورية و لبنان باستخدام اختبار التفاعل المتسلسل للبوليمراز مع النسخ العكسي المتعدد

Abstract EN

Polymyxa betae Keskin is considered the only vector of Beet necrotic yellow vein virus (BNYVV) and Beet soil borne virus (BSBV).

In addition, P.

betae is a vector of other viruses such as Beet soil borne mosaic virus (BSBMV) and Beet virus Q (BVQ).

Six soil samples were collected from different locations of sugar beet fields previously infected with Rhizomania in Syria and Lebanon.

Detection of resting spores of P.

betae was carried out in the roots of sugar beet using scanning electron microscopy with multiple dyes and magnifications up to 6000 x.

Total RNA was extracted from sugar beet samples infected with P.

betae and subjected to multiplex-PCR test using specific primers of BNYVV, BSBV, BSBMV and BVQ.

BNYVV was detected in Homs (Talbisah), Al Ghab (Ein Elkorum) and Lebanon (Maallaka) samples; BSBV was found in Homs (Talbisah) and Lebanon (Maallaka) samples; whereas BSBMV was only detected in Lebanon (Al rawda).