دراسة مقارنة لمستضدات طوري الليشمانيا الجلدية المدارية أمامي السوط Promastigote و عديم السوط Amastigote
Other Title(s)
Comparative study for Promastigote and Amastigote stages antigens of cutaneous leishmania tropica
Joint Authors
قويدر، محمود
العقلة، سعاد
درويش، بثينة
Source
مجلة جامعة دمشق للعلوم الأساسية
Issue
Vol. 28, Issue 1 (30 Jun. 2012), pp.439-460, 22 p.
Publisher
Publication Date
2012-06-30
Country of Publication
Syria
No. of Pages
22
Main Subjects
Abstract EN
The parasitic antigens are of great interest in immune responses and in the strategies for developing vaccines.
The aim of this study was to identify the major antigens which are expressed in the two leishmania tropica stages, promastigote & amastigote, employing the western blot analysis using the sera collected from mice immunized with the above mentioned parasite stages' total protein.
The parasites were harvested from patients with cutaneous leishmaniasis and cultured in N.N.N medium at 26°C to be transformed into promastigotes, then were passed to liquid medium RPMI-1640.
The extracellular amastigotes were produced by incubating the promastigotes at 37°C, while the intracellular amastigotes were prepared by infecting the murine peritoneal macrophages with stationary phase promastigotes.
The total proteins were separated using suitable lysis buffer, the protein concentrations were estimated by the Bradford assay.
The molecular masses of these proteins were evaluated using SDS-PAGE.
The comparison of antigens in promastigotes, extracellular amastigotes and intracellular amastigotes, showed three types of antigens; similar antigens in the two stages their molecular weights are (67-63), 57, 36 and 28 KDa, amastigotes specific antigens 50, 44 and 34 KDa, and promastigotes specific antigens with the molecular weights 55 and 43 KDa.
In addition, this study also presented differences in the antigens between extracellular and intracellular amastigotes.
American Psychological Association (APA)
درويش، بثينة وقويدر، محمود والعقلة، سعاد. 2012. دراسة مقارنة لمستضدات طوري الليشمانيا الجلدية المدارية أمامي السوط Promastigote و عديم السوط Amastigote. مجلة جامعة دمشق للعلوم الأساسية،مج. 28، ع. 1، ص ص. 439-460.
https://search.emarefa.net/detail/BIM-750319
Modern Language Association (MLA)
قويدر، محمود....[و آخرون]. دراسة مقارنة لمستضدات طوري الليشمانيا الجلدية المدارية أمامي السوط Promastigote و عديم السوط Amastigote. مجلة جامعة دمشق للعلوم الأساسية مج. 28، ع. 1 (2012)، ص ص. 439-460.
https://search.emarefa.net/detail/BIM-750319
American Medical Association (AMA)
درويش، بثينة وقويدر، محمود والعقلة، سعاد. دراسة مقارنة لمستضدات طوري الليشمانيا الجلدية المدارية أمامي السوط Promastigote و عديم السوط Amastigote. مجلة جامعة دمشق للعلوم الأساسية. 2012. مج. 28، ع. 1، ص ص. 439-460.
https://search.emarefa.net/detail/BIM-750319
Data Type
Journal Articles
Language
Arabic
Notes
يتضمن مراجع ببليوجرافية : ص. 459-460
Record ID
BIM-750319