دراسة مقارنة لمستضدات طوري الليشمانيا الجلدية المدارية أمامي السوط Promastigote و عديم السوط Amastigote

Abstract EN

The parasitic antigens are of great interest in immune responses and in the strategies for developing vaccines.

The aim of this study was to identify the major antigens which are expressed in the two leishmania tropica stages, promastigote & amastigote, employing the western blot analysis using the sera collected from mice immunized with the above mentioned parasite stages' total protein.

The parasites were harvested from patients with cutaneous leishmaniasis and cultured in N.N.N medium at 26°C to be transformed into promastigotes, then were passed to liquid medium RPMI-1640.

The extracellular amastigotes were produced by incubating the promastigotes at 37°C, while the intracellular amastigotes were prepared by infecting the murine peritoneal macrophages with stationary phase promastigotes.

The total proteins were separated using suitable lysis buffer, the protein concentrations were estimated by the Bradford assay.

The molecular masses of these proteins were evaluated using SDS-PAGE.

The comparison of antigens in promastigotes, extracellular amastigotes and intracellular amastigotes, showed three types of antigens; similar antigens in the two stages their molecular weights are (67-63), 57, 36 and 28 KDa, amastigotes specific antigens 50, 44 and 34 KDa, and promastigotes specific antigens with the molecular weights 55 and 43 KDa.

In addition, this study also presented differences in the antigens between extracellular and intracellular amastigotes.