Phenotypic and genotypic identification of flouroquinolones resistant escherichia coli isolated from diseased chicken in Suez Canal District
Other Title(s)
التعريف الظاهري و الجيني للميكروب القولوني المقاوم للفلوروكينولون المعزول من الدجاج المصاب في منطقة قناة السويس
Joint Authors
al-Shadhili, Daliyah Abd al-Rahman
Nasif, Suad Abd al-Aziz Abd al-Wanis
Mahmud, Fadwa Fathi
Source
Kafr El-Sheikh Veterinary Medical Journal
Issue
Vol. 12, Issue 1 (30 Jun. 2014), pp.1-22, 22 p.
Publisher
Kafr El-Sheikh University Faculty of Veterinary Medicine
Publication Date
2014-06-30
Country of Publication
Egypt
No. of Pages
22
Main Subjects
Abstract EN
A total of 70 Escherichia coli strains were isolated from samples (liver and heart) collected from diseased chickens (n=105) in about 21 commercial farms located in Suez Canal district of Egypt during May-July 2012.
We investigated the susceptibility to Flouroquinolones (Ciprofloxacin, Enrofloxacin and Norfloxacin) and 9 other antibacterials (Gentamicin, Streptomycin, Doxycyclin, Amoxicillin, Ampicillin, Cephazolin, Cephradine, Lincomycin, Florfenicole) by disc diffusion method and measured the MIC of ciprofloxacin and enrofloxacin.
Resulted flouroquinolones resistant E.coli isolates were then serotyped, and investigated for the prevalence of plasmidmediated fluoroquinolone resistance (PMQR) genes (qnrA, qnrB, qnrS, aac(6`)-Ib-cr, and qepA) by polymerase chain reaction.
Among the E.coli isolates, 72.8% (51/70) were resistant to Ciprofloxacin and Enrofloxacin with MIC ≥ 4 and ≥ 2 (μg/ml) respectively.
These isolates were resistant also to at least 6 other investigated antibacterials in the study for susceptibility/resistance.
Among the resistant 51 E.coli strains, 36 (70.5%) were positive for PMQR genes, 27 (52.9%) were positive for at least one gene of the qnr genes investigated in the study, 16 (31.37%) were positive for qnrA, qnrB and qnrS separately, 20 (39.2%) were positive to aac(6`)-Ib-cr, 35 (68.6%) were positive to qepA, while 4 (7.8%) harbored all the 5 investigated genes.
The resistance of E.
coli to flouroquinolones has been increasing in the past twenty years but unfortunately no enough data about isolates harboring PMQR genes in Egypt either in human or poultry field.
Further investigations concerning the antimicrobial resistance problem in human and veterinary field in Egypt is recommended as a surveillance program is a must.
American Psychological Association (APA)
al-Shadhili, Daliyah Abd al-Rahman& Nasif, Suad Abd al-Aziz Abd al-Wanis& Mahmud, Fadwa Fathi. 2014. Phenotypic and genotypic identification of flouroquinolones resistant escherichia coli isolated from diseased chicken in Suez Canal District. Kafr El-Sheikh Veterinary Medical Journal،Vol. 12, no. 1, pp.1-22.
https://search.emarefa.net/detail/BIM-761326
Modern Language Association (MLA)
al-Shadhili, Daliyah Abd al-Rahman…[et al.]. Phenotypic and genotypic identification of flouroquinolones resistant escherichia coli isolated from diseased chicken in Suez Canal District. Kafr El-Sheikh Veterinary Medical Journal Vol. 12, no. 1 (2014), pp.1-22.
https://search.emarefa.net/detail/BIM-761326
American Medical Association (AMA)
al-Shadhili, Daliyah Abd al-Rahman& Nasif, Suad Abd al-Aziz Abd al-Wanis& Mahmud, Fadwa Fathi. Phenotypic and genotypic identification of flouroquinolones resistant escherichia coli isolated from diseased chicken in Suez Canal District. Kafr El-Sheikh Veterinary Medical Journal. 2014. Vol. 12, no. 1, pp.1-22.
https://search.emarefa.net/detail/BIM-761326
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 15-22
Record ID
BIM-761326