The effect of coumarin derivatives (compounds) on the Vibrio cholerae isolates from different clinical Iraqi sources
Other Title(s)
تأثير مركبات الكومارين على عزلات من بكتريا Vibrio cholera المعزولة من مصادر سريرية عراقية مختلفة
Joint Authors
Abdul, Fatimah Ramadan
Tahir, Nihad Abd
Battah, Inam Hamid
Hasan, Ashraf Sami
Source
Iraqi Journal of Pharmaceutical Sciences
Issue
Vol. 26, Issue 1 (30 Jun. 2017), pp.32-39, 8 p.
Publisher
University of Baghdad College of Pharmacy
Publication Date
2017-06-30
Country of Publication
Iraq
No. of Pages
8
Main Subjects
Topics
Abstract EN
From a large number of bacterial samples collected from different hospital in Iraq in central health laboratory ,only ten isolates were identified primary as Vibrio.
A number of morphology and biochemical test were carried out to complete this identification that showed all bacterial isolates were related to Vibrio cholerae .In this study all Vibrio isolates were investigated for Bio typing and the result showed that all (10) isolate were related to (Eltor biotypes) .Also, the susceptibility test towards eight antibiotics were carried out .
Results shows that ciprofloxacin , Norfloxacin, Erythromycin, Ampicillin, ceftriaxone and Amikacin were the most effective antibiotics and their resistance percentage were 20%,20 %,20, 20,30% and 30% respectively ,While Chloramphenicol and Co- trimoxazole were less effective and their resistance percentage were 90 % both of them.
Three (3,5,6) isolates V.
cholerae were selected depending on results of antibiotics sensitivity tests as showed multiple –antibiotics resistance(100 %).
Then tested to study the effect of coumarin derivatives compounds (1, 2, 3 ) which showed inhibitory effect on V.
cholera (3,5,6) isolates and the compound ( 3 ) showed the highest antibacterial activity of (12,15,14 mm) of inhibition zone diameter against V.
cholera (3,5,6) isolates respectively.
Also, these Iraqi isolates (3,5,6) used to test the effect of acridine orange (0.1%) as acuring agent , the results showed that all (3) isolates V.
cholerae were sensitive to (ciprofloxacin, ceftrixone and Norfloxacin), While the rest were resistance to remained five antibiotics.
The results of Agarose –gel electrophoresis of both normal V.
cholerae (3,5,6) and cured isolates showed the presence of chromosomal and plasmid DNA bands in the normal case ,While only chromosomal DNA bands occur with V.
cholerae (isolate 8) treated with an acridine orange at concentration of (10-2 to 10-4).
American Psychological Association (APA)
Abdul, Fatimah Ramadan& Tahir, Nihad Abd& Hasan, Ashraf Sami& Battah, Inam Hamid. 2017. The effect of coumarin derivatives (compounds) on the Vibrio cholerae isolates from different clinical Iraqi sources. Iraqi Journal of Pharmaceutical Sciences،Vol. 26, no. 1, pp.32-39.
https://search.emarefa.net/detail/BIM-768891
Modern Language Association (MLA)
Hasan, Ashraf Sami…[et al.]. The effect of coumarin derivatives (compounds) on the Vibrio cholerae isolates from different clinical Iraqi sources. Iraqi Journal of Pharmaceutical Sciences Vol. 26, no. 1 (2017), pp.32-39.
https://search.emarefa.net/detail/BIM-768891
American Medical Association (AMA)
Abdul, Fatimah Ramadan& Tahir, Nihad Abd& Hasan, Ashraf Sami& Battah, Inam Hamid. The effect of coumarin derivatives (compounds) on the Vibrio cholerae isolates from different clinical Iraqi sources. Iraqi Journal of Pharmaceutical Sciences. 2017. Vol. 26, no. 1, pp.32-39.
https://search.emarefa.net/detail/BIM-768891
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 37-39
Record ID
BIM-768891