Virulence genes profile of Pseudomonas aeruginosa local isolates from burns and wounds

Abstract EN

In this study a total of 111 swabs were collected from 50 patients suffering from burns and wounds between the age 9 months -69 years from both gender , the specimens were collected from Educational Al-Yarmouk and AL Kadhimiya hospitals in Baghdad during the period between October-December\2014, All the samples were identified by biochemical tests , API 20 E and Vitak -2 .

From 111 specimens, 31 isolates were Pseudomonas aeruginosa , 33 isolates were Escherichia coli and 10 isolates were Klebsiella spp, .

Drug susceptibility tests for the 31 isolates of Pseudomonas aeruginosa were studied by the disk diffusion method against 5 antibiotics and the result showed the percentage of resistance: Carbenicillin 100%, Ticarcillin 61 %, Pipracillin 32 % ,Cefprozil 93% , Colistin 84% .

The DNA samples of the 31 isolates of Pseudomonas.aeruginosa were extracted by DNA extraction kit , the concentration for all 31 DNA samples were between 60- 110 ng /ul and the purity were between 1.8-2 .PCR (Polymerase Chain Reactin) was used for screening the virulence factor genes , protein synthesis inhibition genes (exo A , las B , exoU ) of the thirty one isolates of Pseudomonas aeruginosa .

The result showed that 27 ( 87%) isolates were positive for exo A and las B genes, 4 ( 13%) were negative, , from 25 burn isolates was 23 (92%) positive isolates and from 6 wound isolates was 4 (66%) positives isolates.

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For the exoU gene the result showed that 17 ( 55%) were positive , 14 ( 45% )were negative , From 25 burn isolates the presence of exoU was seen in 15 ( 60 %), while from 6 wound isolates was 2 (33 %).