Effects of L-Carnitine on morphology and antioxidant enzymes and DNA integrity of cryopreserved buffalo spermatozoa
العناوين الأخرى
دراسة تأثير ل-كارنتين على قدرة حيامن الجاموس للتجميد، أنزيمات الأكسدة و التغيرات في تركيب المحتوى النووي
المؤلفون المشاركون
Ibrahim, Ibtihal A.
Duhyrij, Rajab Abd al-Munim
Husayn, Mamduh S.
Hasan, Hani Muhammad Sami
Badr, Majdi Ramadan
المصدر
Assiut Veterinary Medical Journal
العدد
المجلد 63، العدد 152 (31 يناير/كانون الثاني 2017)، ص ص. 173-182، 10ص.
الناشر
تاريخ النشر
2017-01-31
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
العلوم الطبيعية والحياتية (متداخلة التخصصات)
الملخص EN
Cryopreservation induces sub lethal damage to the spermatozoa, thereby reduce their fertile life.
There are some biochemical additives that may enhance buffalo semen freezability; L-carnitine is one of these biochemical semen additives.
Till now, the exact effects of L-carnitine on buffalo semen processing outcomes haven’t been discovered.
The current study aimed to clarify L-carnitine roles during buffalo semen cryopreservation.
Semen was cryopreserved in tris-based extender supplemented with different concentrations of L- carnitine (0.01, 0.05 and 0.1mg/ml)Vs.Tris-based extender only (control).
Then they were processed to cryopreservation and thawing to assess different semen characteristics.
Cryopreserved semen was assessed for percentage post- thawing motility, acrosomal and plasma membrane integrity, viability, DNA damage, antioxidant enzymes concentration, lipid peroxidation, in vitro fertilizing potentials and conception rate.
Current results indicated that addition of 0.05 mg/ml L- carnitine to semen extender significantly (P<0.05) improved post-thawing motility, viability and acrosomal integrity (63.33±9.28 %, 133.33±9.40 and 12.33± 2.02 %, respectively) compared with control (43.33±6.01 %, 74.16± 10.93 and 24.67±2.03 %, respectively).
Moreover, 0.05mg/ml L- carnitine significantly increased (P<0.05) total antioxidant capacity (TAC), superoxide dismutase (SOD) and glutathione peroxidase (GPx) concentrations (0.50±0.07 mμ/ml, 73.67±5.37 U/ml and 106.66±12.03 U/L, respectively) with respect to the control (0.19±0.01 mμ/ml, 27.33±3.49 U/ml and52.33±4.09 U/L, respectively).
Furthermore, 0.05mg/ml L- carnitine significantly decreased (P<0.05) lipid peroxidation of the cryopreserved spermatozoa compared with the control semen (11.00±1.73 vs 24.82±4.90nmol/ml).
Likewise, at this concentration sperm DNA damage, tail length and tail moment of the cryopreserved semen significantly (P<0.05) reduced (1.87±0.36 %, 1.83±0.33 μm and 3.72±1.44, respectively) compared with control (3.47±0.13 %, 3.48±0.17 μm and 11.91±0.87, respectively).
Additionally, 0.05 mg/ml L- carnitine significantly (P<0.05) improved in vitro fertilization rate (57.45%) and conception rate (61.76%) compared with the control (33.33 and 37.93%, respectively).
In conclusion the use of 0.05 mg/ml L-carnitine in the freezing extender improves DNA integrity through enhancing the antioxidant defense of buffalo sperm cells and decreasing the rate of lipid peroxidation.
Therefore, L-carnitine may improve sperm cryopreservation quality, reduce cryodamage and improve sperm fertilizing potential.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Badr, Majdi Ramadan& Ibrahim, Ibtihal A.& Duhyrij, Rajab Abd al-Munim& Husayn, Mamduh S.& Hasan, Hani Muhammad Sami. 2017. Effects of L-Carnitine on morphology and antioxidant enzymes and DNA integrity of cryopreserved buffalo spermatozoa. Assiut Veterinary Medical Journal،Vol. 63, no. 152, pp.173-182.
https://search.emarefa.net/detail/BIM-781578
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Badr, Majdi Ramadan…[et al.]. Effects of L-Carnitine on morphology and antioxidant enzymes and DNA integrity of cryopreserved buffalo spermatozoa. Assiut Veterinary Medical Journal Vol. 63, no. 152 (Jan. 2017), pp.173-182.
https://search.emarefa.net/detail/BIM-781578
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Badr, Majdi Ramadan& Ibrahim, Ibtihal A.& Duhyrij, Rajab Abd al-Munim& Husayn, Mamduh S.& Hasan, Hani Muhammad Sami. Effects of L-Carnitine on morphology and antioxidant enzymes and DNA integrity of cryopreserved buffalo spermatozoa. Assiut Veterinary Medical Journal. 2017. Vol. 63, no. 152, pp.173-182.
https://search.emarefa.net/detail/BIM-781578
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 180-182
رقم السجل
BIM-781578
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر