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Effects of L-Carnitine on morphology and antioxidant enzymes and DNA integrity of cryopreserved buffalo spermatozoa
Other Title(s)
دراسة تأثير ل-كارنتين على قدرة حيامن الجاموس للتجميد، أنزيمات الأكسدة و التغيرات في تركيب المحتوى النووي
Joint Authors
Ibrahim, Ibtihal A.
Duhyrij, Rajab Abd al-Munim
Husayn, Mamduh S.
Hasan, Hani Muhammad Sami
Badr, Majdi Ramadan
Source
Assiut Veterinary Medical Journal
Issue
Vol. 63, Issue 152 (31 Jan. 2017), pp.173-182, 10 p.
Publisher
Assiut University Faculty of Veterinary Medicine
Publication Date
2017-01-31
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Abstract EN
Cryopreservation induces sub lethal damage to the spermatozoa, thereby reduce their fertile life.
There are some biochemical additives that may enhance buffalo semen freezability; L-carnitine is one of these biochemical semen additives.
Till now, the exact effects of L-carnitine on buffalo semen processing outcomes haven’t been discovered.
The current study aimed to clarify L-carnitine roles during buffalo semen cryopreservation.
Semen was cryopreserved in tris-based extender supplemented with different concentrations of L- carnitine (0.01, 0.05 and 0.1mg/ml)Vs.Tris-based extender only (control).
Then they were processed to cryopreservation and thawing to assess different semen characteristics.
Cryopreserved semen was assessed for percentage post- thawing motility, acrosomal and plasma membrane integrity, viability, DNA damage, antioxidant enzymes concentration, lipid peroxidation, in vitro fertilizing potentials and conception rate.
Current results indicated that addition of 0.05 mg/ml L- carnitine to semen extender significantly (P<0.05) improved post-thawing motility, viability and acrosomal integrity (63.33±9.28 %, 133.33±9.40 and 12.33± 2.02 %, respectively) compared with control (43.33±6.01 %, 74.16± 10.93 and 24.67±2.03 %, respectively).
Moreover, 0.05mg/ml L- carnitine significantly increased (P<0.05) total antioxidant capacity (TAC), superoxide dismutase (SOD) and glutathione peroxidase (GPx) concentrations (0.50±0.07 mμ/ml, 73.67±5.37 U/ml and 106.66±12.03 U/L, respectively) with respect to the control (0.19±0.01 mμ/ml, 27.33±3.49 U/ml and52.33±4.09 U/L, respectively).
Furthermore, 0.05mg/ml L- carnitine significantly decreased (P<0.05) lipid peroxidation of the cryopreserved spermatozoa compared with the control semen (11.00±1.73 vs 24.82±4.90nmol/ml).
Likewise, at this concentration sperm DNA damage, tail length and tail moment of the cryopreserved semen significantly (P<0.05) reduced (1.87±0.36 %, 1.83±0.33 μm and 3.72±1.44, respectively) compared with control (3.47±0.13 %, 3.48±0.17 μm and 11.91±0.87, respectively).
Additionally, 0.05 mg/ml L- carnitine significantly (P<0.05) improved in vitro fertilization rate (57.45%) and conception rate (61.76%) compared with the control (33.33 and 37.93%, respectively).
In conclusion the use of 0.05 mg/ml L-carnitine in the freezing extender improves DNA integrity through enhancing the antioxidant defense of buffalo sperm cells and decreasing the rate of lipid peroxidation.
Therefore, L-carnitine may improve sperm cryopreservation quality, reduce cryodamage and improve sperm fertilizing potential.
American Psychological Association (APA)
Badr, Majdi Ramadan& Ibrahim, Ibtihal A.& Duhyrij, Rajab Abd al-Munim& Husayn, Mamduh S.& Hasan, Hani Muhammad Sami. 2017. Effects of L-Carnitine on morphology and antioxidant enzymes and DNA integrity of cryopreserved buffalo spermatozoa. Assiut Veterinary Medical Journal،Vol. 63, no. 152, pp.173-182.
https://search.emarefa.net/detail/BIM-781578
Modern Language Association (MLA)
Badr, Majdi Ramadan…[et al.]. Effects of L-Carnitine on morphology and antioxidant enzymes and DNA integrity of cryopreserved buffalo spermatozoa. Assiut Veterinary Medical Journal Vol. 63, no. 152 (Jan. 2017), pp.173-182.
https://search.emarefa.net/detail/BIM-781578
American Medical Association (AMA)
Badr, Majdi Ramadan& Ibrahim, Ibtihal A.& Duhyrij, Rajab Abd al-Munim& Husayn, Mamduh S.& Hasan, Hani Muhammad Sami. Effects of L-Carnitine on morphology and antioxidant enzymes and DNA integrity of cryopreserved buffalo spermatozoa. Assiut Veterinary Medical Journal. 2017. Vol. 63, no. 152, pp.173-182.
https://search.emarefa.net/detail/BIM-781578
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 180-182
Record ID
BIM-781578