High perfarmance liquid chromatographic determination of ascorbic acid in pharmaceutical preparations, fruit juices and human serum
Other Title(s)
تقدير حامض الأسكوربيك في المستحضرات الصيدلانية و عصائر الفاكهة و مصل دم الانسان باستخدام طريقة كروماتوغرافيا السائل عالي الأداء
Joint Authors
Mahmud, Hana Shukr
Ahmad, Rabah R.
Source
Issue
Vol. 27, Issue 3 (30 Jun. 2018), pp.79-92, 14 p.
Publisher
University of Mosul College of Science
Publication Date
2018-06-30
Country of Publication
Iraq
No. of Pages
14
Main Subjects
Topics
Abstract EN
A high performance liquid chromatographic method for determination of ascorbic acid is described.
The analysis is achieved using cosmosil 5C18-MS-II column (250 mm x 4.6 mm i.d., 5 μm particle size) at room temperature.
The mobile phase used was acetonitrile (ACN), dichloromethane (DCM), and 0.25% K2HPO4 solution in the ratio (90:5:5)(V:V:V).
The flow rate is set to 1.0ml.min-1 with UV-detection at 246 nm.
Beer's law is obeyed over the concentration range 0.05-30 μg.ml-1.
The method is accurate (relative error % is less than 0.12%), precise (RSD better than ±1.25%), successfully applied to the determination of ascorbic acidin its pharmaceutical preparations, fruit juices, and human serum.
American Psychological Association (APA)
Mahmud, Hana Shukr& Ahmad, Rabah R.. 2018. High perfarmance liquid chromatographic determination of ascorbic acid in pharmaceutical preparations, fruit juices and human serum. Rafidain Journal of Science،Vol. 27, no. 3, pp.79-92.
https://search.emarefa.net/detail/BIM-901461
Modern Language Association (MLA)
Mahmud, Hana Shukr& Ahmad, Rabah R.. High perfarmance liquid chromatographic determination of ascorbic acid in pharmaceutical preparations, fruit juices and human serum. Rafidain Journal of Science Vol. 27, no. 3 (2018), pp.79-92.
https://search.emarefa.net/detail/BIM-901461
American Medical Association (AMA)
Mahmud, Hana Shukr& Ahmad, Rabah R.. High perfarmance liquid chromatographic determination of ascorbic acid in pharmaceutical preparations, fruit juices and human serum. Rafidain Journal of Science. 2018. Vol. 27, no. 3, pp.79-92.
https://search.emarefa.net/detail/BIM-901461
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 91-92
Record ID
BIM-901461