High perfarmance liquid chromatographic determination of ascorbic acid in pharmaceutical preparations, fruit juices and human serum

Abstract EN

A high performance liquid chromatographic method for determination of ascorbic acid is described.

The analysis is achieved using cosmosil 5C18-MS-II column (250 mm x 4.6 mm i.d., 5 μm particle size) at room temperature.

The mobile phase used was acetonitrile (ACN), dichloromethane (DCM), and 0.25% K2HPO4 solution in the ratio (90:5:5)(V:V:V).

The flow rate is set to 1.0ml.min-1 with UV-detection at 246 nm.

Beer's law is obeyed over the concentration range 0.05-30 μg.ml-1.

The method is accurate (relative error % is less than 0.12%), precise (RSD better than ±1.25%), successfully applied to the determination of ascorbic acidin its pharmaceutical preparations, fruit juices, and human serum.