Serotyping, virulence gene expression and phenotypic characterization of E. coli O157 : H7 in colibacillosis affecting buffalo calves in Basra governorate
Other Title(s)
التصنيف المصلي، التعبير الجيني للضراوة و التوصيف المظهري للإشريكية القولونية O157 : H7 المعزولة من عجول الجاموس في محافظة البصرة
Joint Authors
Salim, Yusuf Ahmad
Imad, Isra
al-Dirawi, Husayn Ali Naji
Salih, Wisam Mundhir Muhammad
Sayhud, Muayyad Hannun
Source
Iraqi Journal of Veterinary Sciences
Issue
Vol. 33, Issue 2 (31 Dec. 2019), pp.447-451, 5 p.
Publisher
University of Mosul College of Veterinary Medicine
Publication Date
2019-12-31
Country of Publication
Iraq
No. of Pages
5
Main Subjects
Topics
Abstract EN
The objective of the current study was to detect the clinical signs of colibacillosis in buffalo calves, isolate E.
coli O157:H7, detect its virulence gene eaeA using PCR and estimate its prevalence.
The current study sampled 120 buffalo calves aged 1day to 5 months from the Al- Basra veterinary Hospital and Private veterinary clinic within the Basra province between October 2017 and July 2018.
A total of 100 calves were naturally diarrheic and the other 20 calves served as controls.
The clinical sings in the diarrheic subjects included a significant increase in body temperature, heart rates, respiration rates and capillary refill time as compared to control group.
Other clinical signs included whitish to yellowish watery diarrhea with tincture of blood, anorexia, weakness, depression, weak suck reflex, dry oral mucous membranes, cold extremities, weak peripheral pulse, dehydration and death.
Using phenotypic characterization tools like MacConky agar, EMB agar, biochemical tests and Viteck, 83 out 100 diarrhea samples confirmed E.
coli.
Using CT- SMACT agar, 31 out of 83 E.
coli isolates were E.
coli O157:H7 positive.
The PCR result indicated that 47 out of the 83 isolated E.
coli samples were positive for eaeA virulence gene.
In conclusion, this study is a debut in the report of E.
coli and E.
coli O157:H7 isolation and genes identification in buffalo calves in Iraq.
Therefore, proper prevention and control measures are requisite to curtail the mortality and morbidity rate caused by Colibacillosis.
American Psychological Association (APA)
al-Dirawi, Husayn Ali Naji& Salih, Wisam Mundhir Muhammad& Sayhud, Muayyad Hannun& Imad, Isra& Salim, Yusuf Ahmad. 2019. Serotyping, virulence gene expression and phenotypic characterization of E. coli O157 : H7 in colibacillosis affecting buffalo calves in Basra governorate. Iraqi Journal of Veterinary Sciences،Vol. 33, no. 2, pp.447-451.
https://search.emarefa.net/detail/BIM-917235
Modern Language Association (MLA)
al-Dirawi, Husayn Ali Naji…[et al.]. Serotyping, virulence gene expression and phenotypic characterization of E. coli O157 : H7 in colibacillosis affecting buffalo calves in Basra governorate. Iraqi Journal of Veterinary Sciences Vol. 33, no. 2 (2019), pp.447-451.
https://search.emarefa.net/detail/BIM-917235
American Medical Association (AMA)
al-Dirawi, Husayn Ali Naji& Salih, Wisam Mundhir Muhammad& Sayhud, Muayyad Hannun& Imad, Isra& Salim, Yusuf Ahmad. Serotyping, virulence gene expression and phenotypic characterization of E. coli O157 : H7 in colibacillosis affecting buffalo calves in Basra governorate. Iraqi Journal of Veterinary Sciences. 2019. Vol. 33, no. 2, pp.447-451.
https://search.emarefa.net/detail/BIM-917235
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 451
Record ID
BIM-917235