Molecular detection of NDV by Real-Time PCR in Baghdad

Abstract EN

Newcastle disease virus (NDV) is widely spread in Iraqi chicken that is associated with notable economic losses.

Current study was carried out to identify NDV in the North of Baghdad by employing a Molecular methods based on the Real-Time PCR technique, the primers and the probe also were designed based on the conserved fragment coding matrix protein(M gene).These genes of virus show the highest level of conservation, and therefore the test is adaptable for detection of NDV.

In the current study, 14 clinically suspicious birds believed to be infected with ND, had been chosen from 14 different commercial poultry farms in various areas of North Baghdad brought to the Aurok and Al-Emad Lab.

Baghdad/ Iraq.

The infected birds were examined and the tissue samples collected from ceca tonsils, spleens, trachea, lungs and brains of each farm.

These samples were submitted to examination directly by Real-time PCR.

The Results of the samples collected from 14 poultry flocks were showed positive results in ten samples with the SYBR Green I real-time PCR and the other 4 flocks failed to show any positive results with both gene primers