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Molecular detection of NDV by Real-Time PCR in Baghdad
Other Title(s)
تشخيص جزيئي لمرض النيوكاسل بتقنية سلسلة إنزم البلمرة الآني في بغداد
Joint Authors
Khashshan, Athir Abd al-Hamid
Suud, Mustafa Ali
Hamad, Muhammad Abd Allah
Source
al-Anbar Journal of Veterinary Sciences
Issue
Vol. 11, Issue 1 (30 Jun. 2018), pp.95-99, 5 p.
Publisher
Publication Date
2018-06-30
Country of Publication
Iraq
No. of Pages
5
Main Subjects
Abstract EN
Newcastle disease virus (NDV) is widely spread in Iraqi chicken that is associated with notable economic losses.
Current study was carried out to identify NDV in the North of Baghdad by employing a Molecular methods based on the Real-Time PCR technique, the primers and the probe also were designed based on the conserved fragment coding matrix protein(M gene).These genes of virus show the highest level of conservation, and therefore the test is adaptable for detection of NDV.
In the current study, 14 clinically suspicious birds believed to be infected with ND, had been chosen from 14 different commercial poultry farms in various areas of North Baghdad brought to the Aurok and Al-Emad Lab.
Baghdad/ Iraq.
The infected birds were examined and the tissue samples collected from ceca tonsils, spleens, trachea, lungs and brains of each farm.
These samples were submitted to examination directly by Real-time PCR.
The Results of the samples collected from 14 poultry flocks were showed positive results in ten samples with the SYBR Green I real-time PCR and the other 4 flocks failed to show any positive results with both gene primers
American Psychological Association (APA)
Hamad, Muhammad Abd Allah& Khashshan, Athir Abd al-Hamid& Suud, Mustafa Ali. 2018. Molecular detection of NDV by Real-Time PCR in Baghdad. al-Anbar Journal of Veterinary Sciences،Vol. 11, no. 1, pp.95-99.
https://search.emarefa.net/detail/BIM-917298
Modern Language Association (MLA)
Hamad, Muhammad Abd Allah…[et al.]. Molecular detection of NDV by Real-Time PCR in Baghdad. al-Anbar Journal of Veterinary Sciences Vol. 11, no. 1 (2018), pp.95-99.
https://search.emarefa.net/detail/BIM-917298
American Medical Association (AMA)
Hamad, Muhammad Abd Allah& Khashshan, Athir Abd al-Hamid& Suud, Mustafa Ali. Molecular detection of NDV by Real-Time PCR in Baghdad. al-Anbar Journal of Veterinary Sciences. 2018. Vol. 11, no. 1, pp.95-99.
https://search.emarefa.net/detail/BIM-917298
Data Type
Journal Articles
Language
English
Notes
Record ID
BIM-917298