Detection the effect of smoking and age on total anti- oxidant capacity level and DNA damage of individuals exposure to low dose ionizing radiation
المؤلفون المشاركون
Abbas, Rafid Kazim
al-Daraghi, Wathiq Abbas Hutayt
Sabah, Majid Arshid
المصدر
Iraqi Journal of Biotechnology
العدد
المجلد 18، العدد 1 (30 إبريل/نيسان 2019)، ص ص. 20-31، 12ص.
الناشر
جامعة بغداد معهد الهندسة الوراثية و التقنيات الإحيائية للدراسات العليا
تاريخ النشر
2019-04-30
دولة النشر
العراق
عدد الصفحات
12
التخصصات الرئيسية
الموضوعات
الملخص EN
The aim of this study to detect the total anti- oxidant capacity (TAC) level and DNA damage by use alkaline comet assay in different measurements as biomarker of double and single strand breaks that caused by smoking and relation with age of individuals exposed to ionizing radiation, this study including 100 male blood samples (30-65) years, TAC level were measured as biomarker for detecting the effect of smoking and age of exposed individuals, in this research recorded non- significant increase in total TAC level in blood serum for the non-smoker individuals as compared with smoker, in other hand also we recorded non -significant result of TAC in three age groups (Less than 40, 40-50 and more than 50) years, but the single cell gel electrophoresis ( comet assay) appear highly significant increase ** (P<0.01) with Mean ±SE (18.63± 0.72) and LSD –value (3.386 **) of DNA damage(high damage measurement HD) in smoker individuals when compared with nonsmoking, the present study shows significant difference in comet high damage cells (* P<0.05) for some age groups that be less than 40 and more than 50years is 12.67 and 17.37 respectively, the study results show that the difference increased of comet assay measurements may be caused by smoking , aging and also effect of works environment.
The level of TAC was non more difference in age groups and in smoker individuals or non- smokers .
In conclusion, found that an negative correlation between TAC level and oxidised bases that measured by comet assay (single and double strands breaks), and age.
This study , indicated that can use the different measurement of comet assay as biomarkers to detect influence of age and smoking in peripheral blood lymphocytes DNA.
Also, the results confirmed usefuless of the TAC as biomarker of smoking and aging effect in exposed individuals
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Abbas, Rafid Kazim& al-Daraghi, Wathiq Abbas Hutayt& Sabah, Majid Arshid. 2019. Detection the effect of smoking and age on total anti- oxidant capacity level and DNA damage of individuals exposure to low dose ionizing radiation. Iraqi Journal of Biotechnology،Vol. 18, no. 1, pp.20-31.
https://search.emarefa.net/detail/BIM-947574
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Abbas, Rafid Kazim…[et al.]. Detection the effect of smoking and age on total anti- oxidant capacity level and DNA damage of individuals exposure to low dose ionizing radiation. Iraqi Journal of Biotechnology Vol. 18, no. 1 (Apr. 2019), pp.20-31.
https://search.emarefa.net/detail/BIM-947574
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Abbas, Rafid Kazim& al-Daraghi, Wathiq Abbas Hutayt& Sabah, Majid Arshid. Detection the effect of smoking and age on total anti- oxidant capacity level and DNA damage of individuals exposure to low dose ionizing radiation. Iraqi Journal of Biotechnology. 2019. Vol. 18, no. 1, pp.20-31.
https://search.emarefa.net/detail/BIM-947574
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 29-31
رقم السجل
BIM-947574
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر