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Peripheral Leukocytapheresis Attenuates Acute Lung Injury Inducedby Lipopolysaccharide In Vivo
Joint Authors
He, Zhi-Gao
Huang, Jian
Zhou, Shun-Gang
He, Jing
Chen, Fang-Xiang
Huang, Xian-Kai
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-03-01
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The mortality of acute lung injury and acute respiratory distress syndrome (ALI/ARDS) remains high and efforts for prevention and treatments have shown little improvement over the past decades.
The present study investigated the efficacy and mechanism of leukocytapheresis (LCAP) to partially eliminate peripheral neutrophils and attenuate lipopolysaccharide (LPS)-induced lung injury in dogs.
A total of 24 healthy male mongrel dogs were enrolled and randomly divided into LPS, LCAP and LCAP-sham groups.
All animals were injected with LPS to induce endotoxemia.
The serum levels of leucocytes, neutrophil elastase, arterial blood gas, nuclear factor-kappa B (NF-κB) subunit p65 in lung tissues were measured.
The histopathology and parenchyma apoptosis of lung tissues were examined.
We found that 7, 3, and 7 animals in the LPS, LCAP, and sham-LCAP groups, respectively, developed ALI 36 h after LPS infusion.
The levels of NF-κB p65 in lung tissue, neutrophils and elastase in blood, decreased significantly following LCAP.
LCAP also alleviated apoptosis, and NF-κB p65 in lung tissues.
Collectively, our results show that partial removal of leucocytes from peripheral blood decreases elastase level in serum.
This, in turn, attenuates lung injuries and may potentially decrease the incidence of ALI.
American Psychological Association (APA)
He, Zhi-Gao& Huang, Jian& Zhou, Shun-Gang& He, Jing& Chen, Fang-Xiang& Huang, Xian-Kai. 2012. Peripheral Leukocytapheresis Attenuates Acute Lung Injury Inducedby Lipopolysaccharide In Vivo. Mediators of Inflammation،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-1001202
Modern Language Association (MLA)
He, Zhi-Gao…[et al.]. Peripheral Leukocytapheresis Attenuates Acute Lung Injury Inducedby Lipopolysaccharide In Vivo. Mediators of Inflammation No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-1001202
American Medical Association (AMA)
He, Zhi-Gao& Huang, Jian& Zhou, Shun-Gang& He, Jing& Chen, Fang-Xiang& Huang, Xian-Kai. Peripheral Leukocytapheresis Attenuates Acute Lung Injury Inducedby Lipopolysaccharide In Vivo. Mediators of Inflammation. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-1001202
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1001202