High-Mobility Group Box 1 Induces Calcineurin-Mediated Cell Hypertrophy in Neonatal Rat Ventricular Myocytes
Joint Authors
Su, Fei-fei
Shi, Miao-qian
Guo, Wan-gang
Liu, Xiong-tao
Wang, Hong-tao
Lu, Zi-fan
Zheng, Qiang-sun
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-06-20
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Cardiac hypertrophy is an independent predictor of cardiovascular morbidity and mortality.
In recent years, evidences suggest that high-mobility group box 1 (HMGB1) protein, an inflammatory cytokine, participates in cardiac remodeling; however, the involvement of HMGB1 in the pathogenesis of cardiac hypertrophy remains unknown.
The aim of this study was to investigate whether HMGB1 is sufficient to induce cardiomyocyte hypertrophy and to identify the possible mechanisms underlying the hypertrophic response.
Cardiomyocytes isolated from 1-day-old Sprague-Dawley rats were treated with recombinant HMGB1, at concentrations ranging from 50 ng/mL to 200 ng/mL.
After 24 hours, cardiomyocytes were processed for the evaluation of atrial natriuretic peptide (ANP) and calcineurin A expression.
Western blot and real-time RT-PCR was used to detect protein and mRNA expression levels, respectively.
The activity of calcineurin was also evaluated using a biochemical enzyme assay.
HMGB1 induced cardiomyocyte hypertrophy, characterized by enhanced expression of ANP, and increased protein synthesis.
Meanwhile, increased calcineurin activity and calcineurin A protein expression were observed in cardiomyocytes preconditioned with HMGB1.
Furthermore, cyclosporin A pretreatment partially inhibited the HMGB1-induced cardiomyocyte hypertrophy.
Our findings suggest that HMGB1 leads to cardiac hypertrophy, at least in part through activating calcineurin.
American Psychological Association (APA)
Su, Fei-fei& Shi, Miao-qian& Guo, Wan-gang& Liu, Xiong-tao& Wang, Hong-tao& Lu, Zi-fan…[et al.]. 2012. High-Mobility Group Box 1 Induces Calcineurin-Mediated Cell Hypertrophy in Neonatal Rat Ventricular Myocytes. Mediators of Inflammation،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-1001228
Modern Language Association (MLA)
Su, Fei-fei…[et al.]. High-Mobility Group Box 1 Induces Calcineurin-Mediated Cell Hypertrophy in Neonatal Rat Ventricular Myocytes. Mediators of Inflammation No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-1001228
American Medical Association (AMA)
Su, Fei-fei& Shi, Miao-qian& Guo, Wan-gang& Liu, Xiong-tao& Wang, Hong-tao& Lu, Zi-fan…[et al.]. High-Mobility Group Box 1 Induces Calcineurin-Mediated Cell Hypertrophy in Neonatal Rat Ventricular Myocytes. Mediators of Inflammation. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-1001228
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1001228