Localization and Upregulation of the Nasal Histamine H1 Receptor in Perennial Allergic Rhinitis
Joint Authors
Kanaizumi, Etsuko
Himi, Tetsuo
Shirasaki, Hideaki
Seki, Nobuhiko
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-10-23
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
In the present study, we have investigated the expression of histamine H1 receptor in human turbinates by RT-PCR, western blotting, and immunohistochemistry.
Human turbinates were obtained by turbinectomy from 12 patients with nasal obstruction refractory to medical therapy.
RT-PCR analysis of total RNA extracted from human nasal turbinate, primary cultured human nasal epithelial cells, and nasal vascular endothelial cells demonstrated the expression of histamine H1 receptor mRNA.
About 56 kDa band was detected in human turbinates by western blot analysis using anti-H1 receptor antibody.
The expression level of H1 receptor protein was marked in patients with nasal allergy than in patients with nonallergic rhinitis.
The immunohistochemical study revealed that epithelial cells and vascular endothelial cells showed intense immunoreactivity for histamine H1 receptor.
In addition, the blood vessels in superficial area expressed higher level of H1 receptor immunoreactivity than that in deeper area in the nasal mucosa.
These results may have an important clinical implication for understanding the role of histamine H1 receptor on upper airway diseases such as allergic rhinitis and nonallergic rhinitis.
American Psychological Association (APA)
Shirasaki, Hideaki& Kanaizumi, Etsuko& Seki, Nobuhiko& Himi, Tetsuo. 2012. Localization and Upregulation of the Nasal Histamine H1 Receptor in Perennial Allergic Rhinitis. Mediators of Inflammation،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-1001259
Modern Language Association (MLA)
Shirasaki, Hideaki…[et al.]. Localization and Upregulation of the Nasal Histamine H1 Receptor in Perennial Allergic Rhinitis. Mediators of Inflammation No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-1001259
American Medical Association (AMA)
Shirasaki, Hideaki& Kanaizumi, Etsuko& Seki, Nobuhiko& Himi, Tetsuo. Localization and Upregulation of the Nasal Histamine H1 Receptor in Perennial Allergic Rhinitis. Mediators of Inflammation. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-1001259
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1001259