Adult-Brain-Derived Neural Stem Cells Grafting into a Vein Bridge Increases Postlesional Recovery and Regeneration in a Peripheral Nerve of Adult Pig
Joint Authors
Liard, Olivier
Segura, Stéphanie
Sagui, Emmanuel
Nau, André
Pascual, Aurélie
Cambon, Melissa
Darlix, Jean-Luc
Fusai, Thierry
Moyse, Emmanuel
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-02
Country of Publication
Egypt
No. of Pages
11
Abstract EN
We attempted transplantation of adult neural stem cells (ANSCs) inside an autologous venous graft following surgical transsection of nervis cruralis with 30 mm long gap in adult pig.
The transplanted cell suspension was a primary culture of neurospheres from adult pig subventricular zone (SVZ) which had been labeled in vitro with BrdU or lentivirally transferred fluorescent protein.
Lesion-induced loss of leg extension on the thigh became definitive in controls but was reversed by 45–90 days after neurosphere-filled vein grafting.
Electromyography showed stimulodetection recovery in neurosphere-transplanted pigs but not in controls.
Postmortem immunohistochemistry revealed neurosphere-derived cells that survived inside the venous graft from 10 to 240 post-lesion days and all displayed a neuronal phenotype.
Newly formed neurons were distributed inside the venous graft along the severed nerve longitudinal axis.
Moreover, ANSC transplantation increased CNPase expression, indicating activation of intrinsic Schwann cells.
Thus ANSC transplantation inside an autologous venous graft provides an efficient repair strategy.
American Psychological Association (APA)
Liard, Olivier& Segura, Stéphanie& Sagui, Emmanuel& Nau, André& Pascual, Aurélie& Cambon, Melissa…[et al.]. 2012. Adult-Brain-Derived Neural Stem Cells Grafting into a Vein Bridge Increases Postlesional Recovery and Regeneration in a Peripheral Nerve of Adult Pig. Stem Cells International،Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-1002951
Modern Language Association (MLA)
Liard, Olivier…[et al.]. Adult-Brain-Derived Neural Stem Cells Grafting into a Vein Bridge Increases Postlesional Recovery and Regeneration in a Peripheral Nerve of Adult Pig. Stem Cells International No. 2012 (2012), pp.1-11.
https://search.emarefa.net/detail/BIM-1002951
American Medical Association (AMA)
Liard, Olivier& Segura, Stéphanie& Sagui, Emmanuel& Nau, André& Pascual, Aurélie& Cambon, Melissa…[et al.]. Adult-Brain-Derived Neural Stem Cells Grafting into a Vein Bridge Increases Postlesional Recovery and Regeneration in a Peripheral Nerve of Adult Pig. Stem Cells International. 2012. Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-1002951
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1002951