Small Molecules Greatly Improve Conversion of Human-Induced Pluripotent Stem Cells to the Neuronal Lineage
Joint Authors
Mak, Sally K.
Huang, Y. Anne
Iranmanesh, Shifteh
Vangipuram, Malini
Sundararajan, Ramya
Nguyen, Loan
Langston, J. William
Schüle, Birgitt
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-10
Country of Publication
Egypt
No. of Pages
12
Abstract EN
Efficient in vitro differentiation into specific cell types is more important than ever after the breakthrough in nuclear reprogramming of somatic cells and its potential for disease modeling and drug screening.
Key success factors for neuronal differentiation are the yield of desired neuronal marker expression, reproducibility, length, and cost.
Three main neuronal differentiation approaches are stromal-induced neuronal differentiation, embryoid body (EB) differentiation, and direct neuronal differentiation.
Here, we describe our neurodifferentiation protocol using small molecules that very efficiently promote neural induction in a 5-stage EB protocol from six induced pluripotent stem cells (iPSC) lines from patients with Parkinson’s disease and controls.
This protocol generates neural precursors using Dorsomorphin and SB431542 and further maturation into dopaminergic neurons by replacing sonic hedgehog with purmorphamine or smoothened agonist.
The advantage of this approach is that all patient-specific iPSC lines tested in this study were successfully and consistently coaxed into the neural lineage.
American Psychological Association (APA)
Mak, Sally K.& Huang, Y. Anne& Iranmanesh, Shifteh& Vangipuram, Malini& Sundararajan, Ramya& Nguyen, Loan…[et al.]. 2012. Small Molecules Greatly Improve Conversion of Human-Induced Pluripotent Stem Cells to the Neuronal Lineage. Stem Cells International،Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-1002953
Modern Language Association (MLA)
Mak, Sally K.…[et al.]. Small Molecules Greatly Improve Conversion of Human-Induced Pluripotent Stem Cells to the Neuronal Lineage. Stem Cells International No. 2012 (2012), pp.1-12.
https://search.emarefa.net/detail/BIM-1002953
American Medical Association (AMA)
Mak, Sally K.& Huang, Y. Anne& Iranmanesh, Shifteh& Vangipuram, Malini& Sundararajan, Ramya& Nguyen, Loan…[et al.]. Small Molecules Greatly Improve Conversion of Human-Induced Pluripotent Stem Cells to the Neuronal Lineage. Stem Cells International. 2012. Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-1002953
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1002953